QL9
Modify Date: 2024-01-08 17:00:25
QL9 structure
|
Common Name | QL9 | ||
|---|---|---|---|---|
| CAS Number | 159646-83-0 | Molecular Weight | 1063.21 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C52H74N10O14 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of QL9QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR). |
| Name | QL9 |
|---|
| Description | QL9 (QLSPFPFDL) is a high-affinity alloantigen for the 2C T cell receptor (TCR). |
|---|---|
| Related Catalog | |
| Target |
TCR[1] |
| In Vitro | Mouse T cell clone 2C recognizes two different major histocompatibility (MHC) ligands, the self MHC Kb and the allogeneic MHC Ld. Two distinct peptides, SIY (SIYRYYGL) and QL9 (QLSPFPFDL), act as strong and specific agonists when bind to Kb and Ld, respectively. QL9 binding to MHC Ld is influenced by the majority of peptide side chains, distributed across the entire length of the peptide. Findings with both systems, but QL9-Ld in particular, suggest that many single-residue substitutions, introduced into peptides to improve their binding to MHC and thus their vaccine potential, could impair T cell reactivity due to their dual impact on TCR binding. T cell activation assays are performed to measure effects of peptide SIY and QL9 residues on T cell function[2]. |
| Cell Assay | Wild type 2C and high affinity 2C T cell transfectants m67 and m6 are incubated with Kb- or Ld-positive cells and various concentrations of peptide SIY and QL9 alanine variants. T cell activation is measured by assaying for levels of IL-2 release. Briefly, T cell transfectants (7.5×104) are incubated with T2-Kb (7.5×104) or T2-Ld (7.5×104) along with various concentrations of peptide for 20-24 h at 37 °C and 5% CO2. Supernatant is harvested, and levels of IL-2 are measured in an enzyme-linked immunosorbent assay type format. Results are plotted as percentage of maximal IL-2 release=((A450 (sample)-A450(no peptide))/(Max A450(sample)-A450(no peptide)))×100; signal obtained from no peptide is similar to that obtained for the null peptides MCMV or OVA. Binding curves are generated in GraphPad Prism by plotting the percentage of maximal IL-2 release against peptide concentration. The concentrations of peptide yielding 50% maximal IL-2 release (SD50) are calculated using non-linear regression (sigmoidal fitting; GraphPad Prism) of the activation curves[2]. |
| References |
| Molecular Formula | C52H74N10O14 |
|---|---|
| Molecular Weight | 1063.21 |
| Storage condition | 2-8℃ |
- Shanghai Nianxing Industrial Co., Ltd
- China
- Product Name: QL9?
- Price: Negotiable
- Purity: 98.0%
- Delivery: 10 Day
- Contact: Tom
- Email: sales@echemcloud.com
- Taiwan Oyi peptide Co. Ltd
- China
- Product Name: QL9
- Price: $Inquiry/1mg $Inquiry/100kg
- Purity: 98.0%
- Delivery: 1 Day
- Contact: Vinnie
- Email: Jamie@api-made.com
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Price: ￥1200/1 mg
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