C6 NBD Galactosylceramide (d18:1/6:0)
Modify Date: 2024-07-21 20:59:56
C6 NBD Galactosylceramide (d18:1/6:0) structure
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Common Name | C6 NBD Galactosylceramide (d18:1/6:0) | ||
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| CAS Number | 170212-26-7 | Molecular Weight | 737.881 | |
| Density | 1.3±0.1 g/cm3 | Boiling Point | N/A | |
| Molecular Formula | C36H59N5O11 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of C6 NBD Galactosylceramide (d18:1/6:0)C6 NBD galactosylceramide is an active derivative of galactosylceramide that is tagged with fluorescent C6 nitrobenzoxadiazole (C6 NBD). C6 NBD galactosylceramide can be used as a substrate for neutral β-glycosylceramidase (GCase) to study intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525)[1]. |
| Name | N-[(2S,3R,4E)-1-(β-D-Galactopyranosyloxy)-3-hydroxy-4-octadecen-2-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide |
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| Synonym | More Synonyms |
| Description | C6 NBD galactosylceramide is an active derivative of galactosylceramide that is tagged with fluorescent C6 nitrobenzoxadiazole (C6 NBD). C6 NBD galactosylceramide can be used as a substrate for neutral β-glycosylceramidase (GCase) to study intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525)[1]. |
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| Related Catalog | |
| In Vitro | C6-NBD-glucosylceramide (4 μM) is transported to the Golgi apparatus in HT29 cells[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2]. Transcytosis of Exogenous C6-NBD-GaICer after Endocytosis: 1. C6-NBD-glucosylceramide is inserted at 10°C. 2. Cells are rinsed three times with cold HBSS' and incubated at 37°C in HBSS' to allow endocytosis. 3. After 10 min, the probe remaining on the cell surface is removed by two (apical) or three (basal) BSA washes for 20 min at 10°C. 4. One set of filters is used for lipid analysis to quantitate endocytosis. A second set of filters is further incubated for 0.5 or 1 h at 37°C in HBSS' + BSA, to assay for reappearance of intracellular C6-NBD-glucosylceramide on either cell surface. 5. The incubations are followed by a 10°C BSA wash, after which the NBD lipids from the combined apical media, basal media, and the cells were extracted into chloroform/methanol, analyzed by TLC, and quantitated. |
| References |
| Density | 1.3±0.1 g/cm3 |
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| Molecular Formula | C36H59N5O11 |
| Molecular Weight | 737.881 |
| Exact Mass | 737.421082 |
| LogP | 6.14 |
| Index of Refraction | 1.585 |
| Hexanamide, N-[(1S,2R,3E)-1-[(β-D-galactopyranosyloxy)methyl]-2-hydroxy-3-heptadecen-1-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- |
| N-[(2S,3R,4E)-1-(β-D-Galactopyranosyloxy)-3-hydroxy-4-octadecen-2-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide |