Calcium Orange AM
Modify Date: 2024-04-03 09:28:05
Calcium Orange AM structure
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Common Name | Calcium Orange AM | ||
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| CAS Number | 172646-19-4 | Molecular Weight | 1223.22 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C59H62N6O21S | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of Calcium Orange AMCalcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles[1]. |
| Name | Calcium Orange AM |
|---|
| Description | Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm). Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles[1]. |
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| Related Catalog | |
| In Vitro | Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Calcium Orange AM is freshly prepared in dehydrated DMSO before each experiment. 2. Prepare the recording medium for measuring [Ca2+]i in cells consisted of 20 mM HEPES, 115 mM NaCl, 5.4 mM KCl, 0.8 mM MgCl2, 1.8 mM CaCl2, and 13.8 mM D-glucose adjusted to pH 7.4 by adding 1 M KOH. 3. Exchange the culture medium for 1 mL of a recording medium including 10 μM Calcium Orange AM. 4. Incubate cells under conditions of 5% CO2 and 37°C for 40 min. 5. Remove the recording medium, including calcium orange AM from the dish, and add the culture medium after washing it three times with 1 mL of the recording medium. 6. Incubate the cells for 1 h. After incubation, exchange the culture medium for the recording medium again. 7. Detect fluorescence. |
| References |
| Molecular Formula | C59H62N6O21S |
|---|---|
| Molecular Weight | 1223.22 |
- Shanghai Nianxing Industrial Co., Ltd
- China
- Product Name: N/A
- Price: Negotiable
- Purity: 98.0%
- Delivery: 10 Day
- Contact: Huang
- Email: sales@echemcloud.com
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