Phenol Red sodium salt structure
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Common Name | Phenol Red sodium salt | ||
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CAS Number | 34487-61-1 | Molecular Weight | 375.351 | |
Density | 0.972 | Boiling Point | 562.8ºC at 760mmHg | |
Molecular Formula | C19H13NaO5S | Melting Point | 285 °C (dec.)(lit.) | |
MSDS | Chinese USA | Flash Point | 294.2ºC | |
Symbol |
GHS07 |
Signal Word | Warning |
Use of Phenol Red sodium saltPhenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range. |
Name | sodium,4-[3-(4-hydroxyphenyl)-1,1-dioxo-2,1λ6-benzoxathiol-3-yl]phenolate |
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Synonym | More Synonyms |
Description | Phenol Red sodium salt is a water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range. |
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Related Catalog | |
In Vitro | Phenol Red sodium salt is commonly employed as a cell tissue culture pH marker. Under normal conditions, phenol red added to the growth medium will have a pink-red color. Dying cells or overgrowth of contaminants will cause a change in pH resulting in a change in indicator color. Phenol red bears a structural resemblance to some nonsteroidal estrogens and has significant estrogenic activity at the concentrations (15-45 μM) at which it is found in tissue culture media. Phenol red binds to the estrogen receptor of MCF-7 human breast cancer cells and stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in a dose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breast cancer cells[1]. Phenol red is a simple, rapid and inexpensive method for the measurement of hydrogen peroxide produced by cells in culture. The assay is based on the horseradish peroxidase-mediated oxidation of phenol red by hydrogen peroxide which results in the formation of a compound demonstrating increased absorbance at 610 nm[2]. |
Cell Assay | Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2]. |
References |
Density | 0.972 |
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Boiling Point | 562.8ºC at 760mmHg |
Melting Point | 285 °C (dec.)(lit.) |
Molecular Formula | C19H13NaO5S |
Molecular Weight | 375.351 |
Flash Point | 294.2ºC |
Exact Mass | 375.030853 |
PSA | 95.04000 |
LogP | 4.62760 |
Storage condition | Store at RT. |
Stability | Stable. Incompatible with strong oxidizing agents. |
Water Solubility | soluble |
Symbol |
GHS07 |
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Signal Word | Warning |
Hazard Statements | H315-H319-H335 |
Precautionary Statements | P261-P305 + P351 + P338 |
Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
Hazard Codes | Xi:Irritant; |
Risk Phrases | R36/37/38 |
Safety Phrases | S26-S36-S37/39 |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
HS Code | 29349990 |
Precursor 0 | |
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DownStream 1 | |
HS Code | 29349990 |
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Phenolsulfonephthalein sodium salt |
Phenol Red,sodium salt |
PR sodium salt |
phenolsulfonphthalein sodium salt |
phenolsulphophthaleine sodium salt |
UNII-50J9732WBG |
MFCD00066901 |
Phenol, 4,4'-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis-, monosodium salt |
EINECS 252-057-8 |
phenolsulfonephthalein sodium |
Sodium 4-(3-(4-hydroxyphenyl)-1,1-dioxido-3H-benzo[c][1,2]oxathiol-3-yl)phenolate |
Phenolate, 4,4'-(1,1-dioxido-3H-2,1-benzoxathiol-3-ylidene)bis-, sodium salt (1:1) |
phenol red sodium salt |
PHENOL RED,ACS |
Sodium Phenol Red |
sodium hydrogen 4,4'-(3H-2,1-benzoxathiol-3-ylidene)bisphenolate S,S-dioxide |
Phenol Red (sodium salt) |