Spliceostatin A
Modify Date: 2024-01-13 22:03:14
Spliceostatin A structure
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Common Name | Spliceostatin A | ||
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| CAS Number | 391611-36-2 | Molecular Weight | 521.64300 | |
| Density | 1.2±0.1 g/cm3 | Boiling Point | 683.2±55.0 °C at 760 mmHg | |
| Molecular Formula | C28H43NO8 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | 367.0±31.5 °C | |
Use of Spliceostatin ASpliceostatin A, the FR901464 (HY-16212) methylated derivative, is a potent anti-tumor agent. Spliceostatin A inhibits splicing and promotes pre-mRNA accumulation by binding SF3B1. SF3B1 is a subcomplex of U2 small nuclear ribonucleoprotein in the spliceosome. Spliceostatin A induces Apoptosis in chronic lymphocytic leukemia (CLL) cells[1][2][3]. |
| Name | spliceostatin A |
|---|
| Description | Spliceostatin A, the FR901464 (HY-16212) methylated derivative, is a potent anti-tumor agent. Spliceostatin A inhibits splicing and promotes pre-mRNA accumulation by binding SF3B1. SF3B1 is a subcomplex of U2 small nuclear ribonucleoprotein in the spliceosome. Spliceostatin A induces Apoptosis in chronic lymphocytic leukemia (CLL) cells[1][2][3]. |
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| Related Catalog | |
| Target |
IC50: 5.5 nM (wild-type SF3B1), 4.9 nM (SF3B1mutatant)[1] |
| In Vitro | Spliceostatin A (2.5-20 nM; 0-24 h) 以剂量和时间依赖的方式诱导 CLL 细胞出现 Caspase 依赖性凋亡[1]。 Spliceostatin A (10 nM, 20 nM; 24 h) 与 ABT-199 或 ABT-263 有协同作用，增加 IL4/CD40L (10 ng/mL, 300 ng/mL;6 h) 处理后 CLL 细胞的凋亡，并降低相关蛋白磷酸化水平[1]。 Spliceostatin A 抑制正常 B (CD19+) 和 T (CD3+) 淋巴细胞的活力，IC50 分别为 12.1 nM 和 61.7 nM[1]。 Apoptosis Analysis[1] Cell Line: CLL cells Concentration: 0, 2.5 nM, 5 nM, 10 nM, and 20 nM Incubation Time: 0, 6 h, 12 h, and 24 h Result: Increased cell apoptosis. Western Blot Analysis[1] Cell Line: CLL cells Concentration: 10 nM, and 20 nM Incubation Time: 24 h; treated after 10 ng/mL IL4 and/or 300 ng/mL CD40L for 6 h Result: Decreased the level of PARP, phosphorylated STAT6 (pSTAT6), phosphorylated IκBα (pIκBα), Mcl-1L, Bcl-xL , Bcl-2 and Hsc70. |
| References |
| Density | 1.2±0.1 g/cm3 |
|---|---|
| Boiling Point | 683.2±55.0 °C at 760 mmHg |
| Molecular Formula | C28H43NO8 |
| Molecular Weight | 521.64300 |
| Flash Point | 367.0±31.5 °C |
| Exact Mass | 521.29900 |
| PSA | 115.85000 |
| LogP | 2.64 |
| Vapour Pressure | 0.0±4.8 mmHg at 25°C |
| Index of Refraction | 1.539 |
| Precursor 0 | |
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| DownStream 1 | |
CAS#:146478-72-0