Leupeptin

Modify Date: 2024-01-03 13:50:54

Leupeptin structure	Common Name	Leupeptin
	CAS Number	55123-66-5	Molecular Weight	951.18600
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₄₀H₇₈N₁₂O₁₂S	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of Leupeptin

Leupeptin is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin potently inhibits serine, cysteine and threonine proteases. Leupeptin inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3].

Name	leupeptin hemisulfate monohydrate
Synonym	More Synonyms

Description	Leupeptin is a broad-spectrum, membrane-permeable protease inhibitor. Leupeptin potently inhibits serine, cysteine and threonine proteases. Leupeptin inhibits Mpro (the main protease of SARS-CoV-2) and also has anti-inflammatory activity[1][2][3].
Related Catalog	Signaling Pathways >> Metabolic Enzyme/Protease >> Ser/Thr Protease Research Areas >> Infection Research Areas >> Inflammation/Immunology Signaling Pathways >> Metabolic Enzyme/Protease >> Cathepsin
Target	Cathepsin B, Cathepsin H, Cathepsin L, Ser/Thr Protease, Mpro[1][2][3].
In Vitro	Leupeptin (0.06-200 µM; 72 h) 显著抑制 Vero 细胞中 SARS-CoV-2 病毒 RNA (vRNA) 的拷贝数[1] 。Leupeptin 抑制 Vero 细胞中 SARS-CoV-2 的 RNA 水平，EC50 值为 42.34 µM[1] 。Leupeptin 对 Mpro 有一定的抑制活性，其 IC50 值为127.2 µM[1] 。Leupeptin 对人类冠状病毒 229E 株的 IC50 值为 0.4 µg/mL (约 1 µM)[1]。 RT-PCR[1] Cell Line: Vero cells Concentration: 0.06-200 µM Incubation Time: 72 h Result: Significantly decreased copy numbers of SARS-CoV-2 viral RNA (vRNA).
In Vivo	Leupeptin (0, 9, 18, 36 mg/kg; i.p.; single) 具有很好的动物耐受性，并在总组织提取物、溶酶体以及富含自噬体的颗粒部分以剂量依赖的方式显著诱导 LC3b-II 的增加[2]。 Animal Model: C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)[1]. Dosage: 0, 9, 18, 36 mg/kg Administration: Intraperitoneal injection; single Result: Promoted the accumulation of LC3b-II in mouse liver.
References	[1]. Fu L, et al. Mechanism of Microbial Metabolite Leupeptin in the Treatment of COVID-19 by Traditional Chinese Medicine Herbs. mBio. 2021 Oct 26;12(5):e0222021. [2]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42. [3]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.

Molecular Formula	C₄₀H₇₈N₁₂O₁₂S
Molecular Weight	951.18600
Exact Mass	950.55800
PSA	415.52000
LogP	5.18540

CHEMICAL IDENTIFICATION

RTECS NUMBER :: YV4205100

CHEMICAL NAME :: Valeramide, 2-(2-acetamido-4-methylvaleramido)-N-(1-formyl-4-guan idinobutyl)-4-methyl- (S)-

CAS REGISTRY NUMBER :: 55123-66-5

LAST UPDATED :: 198910

DATA ITEMS CITED :: 1

MOLECULAR FORMULA :: C20-H38-N6-O4

MOLECULAR WEIGHT :: 426.64

WISWESSER LINE NOTATION :: MUYZM3YVHMVY1Y1&1&MVYMV1&1Y1&1 S-

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 57600 mg/kg/69W-C

TOXIC EFFECTS :: Tumorigenic - neoplastic by RTECS criteria Lungs, Thorax, or Respiration - tumors Liver - tumors

REFERENCE :: GANNA2 Gann. Japanese Journal of Cancer Research. (Tokyo, Japan) V.1-75, 1907-84. For publisher information, see JJCREP. Volume(issue)/page/year: 71,913,1980

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Gentibiose

Ac-Leu-Leu-Arg-H

N-acetyl-Leu-Leu-argininal

AC-LEU-LEU-ARGINAL 1/2H2SO4 H2O

Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Leupeptin
Price: Click To Check
Purity: 98.0%
Delivery: 10 Day
Contact: Yang
Email: sales@echemcloud.com

DC Chemicals Limited
China
Product Name: Leupeptin
Price: $Inquiry/100mg $Inquiry/250mg $Inquiry/500mg
Purity: 98.0%
Delivery: 3 Day
Contact: Tony Cao
Email: sales@dcchemicals.com

Nanjing Peptide Biotech Ltd
China
Product Name: leupeptin hemisulfate monohydrate
Price: ￥Inquiry/10g ￥Inquiry/100g ￥Inquiry/1kg ￥Inquiry/10kg
Purity: 98.0%
Delivery: 7 Day
Contact: jom
Email: chenglong.fan@njpeptide.com

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: LEUPEPTIN HEMISULFATE MONOHYDRATE
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Delivery: Inquiry
Contact: Ms Wang
Email: enquiry@dycnchem.com

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Leupeptin

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