TWS119

Modify Date: 2024-01-04 14:42:46

TWS119 Structure
TWS119 structure
Common Name TWS119
CAS Number 601514-19-6 Molecular Weight 318.329
Density 1.4±0.1 g/cm3 Boiling Point 646.0±55.0 °C at 760 mmHg
Molecular Formula C18H14N4O2 Melting Point N/A
MSDS Chinese USA Flash Point 344.5±31.5 °C
Symbol GHS07
GHS07
Signal Word Warning

 Use of TWS119


TWS119 is a specific inhibitor of GSK-3β, with an IC50 of 30 nM, and activates the wnt/β-catenin pathway.

 Names

Name 3-[[6-(3-aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy]phenol
Synonym More Synonyms

 TWS119 Biological Activity

Description TWS119 is a specific inhibitor of GSK-3β, with an IC50 of 30 nM, and activates the wnt/β-catenin pathway.
Related Catalog
Target

GSK-3β:30 nM (IC50)

In Vitro TWS119 induces neuronal differentiation in P19 EC cells and primary mouse ESCs. TWS119 binds to GSK-3β with KD of 126 nM, and modulates the activity of the complex, triggering downstream transcriptional events that lead the neuronal induction[1]. TWS119 (< 4 μM) significantly enhances the proliferation and survival of γδT cells via activation of the mammalian target of rapamycin (mTOR) pathway, upregulation of the expression of anti-apoptotic protein Bcl-2 and inhibition of cleaved caspase-3. TWS119 (0-8 μM) induces the generation of CD62L+γδT or CCR5+γδT cell phenotypes. TWS119 (0.5, 1.0 and 2 μM) increases the expression level of granzyme B in a dose-dependent manner. TWS119 also enhances the cytolytic activity of γδT cells against tumour cells in vitro[3].
In Vivo TWS119 (30 mg/kg, i.p.) improves the neurologic function and decreases neurologic deficit dcore in rtPA-treated MCAO rats. TWS119 effectively relieves cerebral edema, and reduces cerebral infarction in rats treated with rtPA. TWS119 also effectively decreases blood-brain barrier permeability in rtPA-Treated MCAO Rats and attenuates rtPA-induced hemorrhage in ischemic brain tissue. Futhermore, TWS119 activates the Wnt/β-Catenin signaling pathway and increases the expression of Claudin-3 and ZO-1[2].
Cell Assay PBMCs are cultured with pamidronate disodium for 8 days and then cells are labelled with or without 1.5 μM carboxyfluorescein succinimidyl ester (CFSE) and CFSE-labelled cells are then seeded in 6-well plates (2.5 × 106 cells/well) followed by treatment with various concentrations of TWS119 for 72 h. The total number of cultured cells is evaluated using an automated cell counter and the γδT cell proliferation is examined by flow cytometry[3].
Animal Admin All rats are randomly divided into four groups as follows: Sham group-rats undego the same surgical procedure, but the filament is not inserted and they receive 1 mL of dimethyl sulfoxide (1 % DMSO in saline); Vehicle group-rats undergo MCAO and receive 1 mL of DMSO; rtPA group-rats underwent MCAO and receive rtPA (10 mg/kg, Actilyse®) at 4 h after MCAO; and rtPA+TWS119 group-rats undergo MCAO and receive intraperitoneal TWS119 (30 mg/kg, dissolved in 1 mL 1 % DMSO) immediately after rtPA injection at 4 h after MCAO[2].
References

[1]. Ding S, et al. Synthetic small molecules that control stem cell fate. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7632-7. Epub 2003 Jun 6.

[2]. Wang W, et al. GSK-3β inhibitor TWS119 attenuates rtPA-induced hemorrhagic transformation and activates the Wnt/β-catenin signaling pathway after acute ischemic stroke in rats. Mol Neurobiol. 2016 Dec;53(10):7028-7036. Epub 2015 Dec 15.

[3]. Chen YQ, et al. Wnt pathway activator TWS119 enhances the proliferation and cytolytic activity of human γδT cells against colon cancer. Exp Cell Res. 2017 Nov 16. pii: S0014-4827(17)30587-6.

 Chemical & Physical Properties

Density 1.4±0.1 g/cm3
Boiling Point 646.0±55.0 °C at 760 mmHg
Molecular Formula C18H14N4O2
Molecular Weight 318.329
Flash Point 344.5±31.5 °C
Exact Mass 318.111664
PSA 97.05000
LogP 3.54
Appearance of Characters light yellow solid
Vapour Pressure 0.0±2.0 mmHg at 25°C
Index of Refraction 1.753
Storage condition Desiccate at +4°C

 Safety Information

Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H302-H319
Precautionary Statements P280-P301 + P312 + P330-P305 + P351 + P338-P337 + P313
RIDADR NONH for all modes of transport

 Synonyms

3-{[6-(3-Aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy}phenol
GSK-3beta Inhibitor XII
TWS 119
Neurogenesis Inducer
Phenol, 3-[[6-(3-aminophenyl)-7H-pyrrolo[2,3-d]pyrimidin-4-yl]oxy]-
TWS119
Top Suppliers:I want be here




Get all suppliers and price by the below link:

TWS119 suppliers


Price: ¥613/10mg

Reference only. check more TWS119 price