5-(Iodoacetamido)fluorescein

Modify Date: 2024-01-03 12:22:25

5-(Iodoacetamido)fluorescein structure	Common Name	5-(Iodoacetamido)fluorescein
	CAS Number	63368-54-7	Molecular Weight	515.25400
	Density	1.95 g/cm3	Boiling Point	788.4ºC at 760 mmHg
	Molecular Formula	C₂₂H₁₄INO₆	Melting Point	265-267ºC (dec.)(lit.)
	MSDS	Chinese USA	Flash Point	430.6ºC

Use of 5-(Iodoacetamido)fluorescein

5-IAF is an idoacetamide derivate of fluoresceine.

Name	5-(Iodoacetamido)fluorescein
Synonym	More Synonyms

Description	5-IAF is an idoacetamide derivate of fluoresceine.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Cancer Dye Reagents
In Vitro	8 µM GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 µM 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 µM 5-IAF[2].
Kinase Assay	HepG2 cells are lysed in 1 mL 2% acetonitrile in water, and transfered to a 1.5 mL centrifuge tube. An additional 200 µL 2% acetonitrile is used to rinse the dish and combined with the cell extract. To measure the free GSH concentration, three aliquots of 80 µL cell extract are immediately transferred to 0.5 mL centrifuge tubes after a quick mix. Then 20 µL 50 µM NAC is added, followed by the addition of 100 µL 400 µM 5-IAF to the mixture. The derivatization is conducted at room temperature in dark for 1 h, after which the mixtures are centrifuged at 9000× g for 5 min to sediment the insoluble proteins. 10 µL supernatant is diluted 100× with water and analyzed immediately by CE-LIF[2].
References	[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7.

Density	1.95 g/cm3
Boiling Point	788.4ºC at 760 mmHg
Melting Point	265-267ºC (dec.)(lit.)
Molecular Formula	C₂₂H₁₄INO₆
Molecular Weight	515.25400
Flash Point	430.6ºC
Exact Mass	514.98700
PSA	116.84000
LogP	4.41510
Index of Refraction	1.781

5-(Iodoacetamido)fluorescein MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases	22-24/25
RIDADR	NONH for all modes of transport

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