JSH-23

Modify Date: 2024-01-02 22:41:01

JSH-23 Structure
JSH-23 structure
Common Name JSH-23
CAS Number 749886-87-1 Molecular Weight 240.343
Density 1.1±0.1 g/cm3 Boiling Point 418.7±40.0 °C at 760 mmHg
Molecular Formula C16H20N2 Melting Point 104.4-105.0℃
MSDS USA Flash Point 245.0±30.9 °C
Symbol GHS05 GHS07 GHS09
GHS05, GHS07, GHS09
Signal Word Danger

 Use of JSH-23


JSH-23 is an NF-κB inhibitor which inhibits NF-κB transcriptional activity with an IC50 of 7.1 μM.

 Names

Name 4-methyl-1-N-(3-phenylpropyl)benzene-1,2-diamine
Synonym More Synonyms

 JSH-23 Biological Activity

Description JSH-23 is an NF-κB inhibitor which inhibits NF-κB transcriptional activity with an IC50 of 7.1 μM.
Related Catalog
Target

NF-κB:7.1 μM (IC50, in RAW 264.7 cells)

In Vitro JSH-23 inhibits lipopolysaccharide (LPS)-induced chromatin condensation in a dose-dependent manner, corresponding to 44±4% inhibition at 3 μM, 63±5% at 10 μM and 93±3% at 30 μM[1]. JSH-23 (5, 10, and 15 μM) significantly reduces mean neuronal migration in LPS-activated cells[2]. Co-treatment of A2780 cells with JSH-23 and clinically ineffective transplatin at their IC50 concentrations (130 μM transplatin and 20 μM JSH-23) for 72 h also causes a more pronounced decrease in cell viability compared to the effects of transplatin or JSH-23 alone[3].
In Vivo JSH-23 (1 and 3 mg/kg, p.o.) significantly reverses the nerve conduction and nerve blood flow deficits seen in diabetic animals and decreases the nerve lipid peroxidation, partially replenishes the depleted levels of GSH in nerve of diabetic rats[4].
Cell Assay Apoptosis is analyzed by 4',6-diamidino-2-phenylindole (DAPI) staining. Macrophages RAW 264.7 incubated with JSH-23 are treated with 1 μg/mL LPS and/or sample for 24 h. The cells are stained with 1 μg/mL DAPI for 30 min at 37°C and then analyzed using fluorescence microscopy with excitation at 300-500 nm. Cells with nuclei containing clearly condensed chromatin or cells with fragmented nuclei are scored as an apoptosis index.
Animal Admin Male Sprague Dawley rats (250-270 g) are used and fed on standard rat diet and water ad libitum. Diabetes is induced by single dose of streptozotocin (STZ, 55 mg/kg, intraperitoneally) in citrate buffer. Blood samples are collected 48 h after STZ administration. Rats with plasma glucose level more than 250 mg/dL are considered as diabetics and are further considered for study. The experimental groups comprised of non-diabetic control rats (ND), diabetic control rats (STZ-D) and diabetic rats treated with two doses of JSH-23 (STZ-D + JSH 1 and STZ-D + JSH 3, respectively, for 1 and 3 mg/kg, orally in 0.5% sodium carboxymethyl cellulose). After 6 weeks of diabetes induction, the drug is administered daily for a period of 2 weeks. The functional, behavioural and biochemical experiments are performed 24 h after the administration of last dose.
References

[1]. Shin, H.M., et al. Inhibitory action of novel aromatic diamine compound on lipopolysaccharide-induced nuclear translocation of NF-kappaB without affecting IkappaB degradation. FEBS Lett, 2004. 571(1-3): p. 50-4.

[2]. Arias-Salvatierra, D., et al. Role of nitric oxide produced by iNOS through NF-kappaB pathway in migration of cerebellar granule neurons induced by Lipopolysaccharide. Cell Signal, 2011. 23(2): p. 425-35.

[3]. Kasparkova, J., et al. Different affinity of nuclear factor-kappa B proteins to DNA modified by antitumor cisplatin and its clinically ineffective trans isomer. FEBS J, 2014. 281(5): p. 1393-408.

[4]. Kumar, A., et al. JSH-23 targets nuclear factor-kappa B and reverses various deficits in experimental diabetic neuropathy: effect on neuroinflammation and antioxidant defence. Diabetes Obes Metab, 2011. 13(8): p. 750-8.

 Chemical & Physical Properties

Density 1.1±0.1 g/cm3
Boiling Point 418.7±40.0 °C at 760 mmHg
Melting Point 104.4-105.0℃
Molecular Formula C16H20N2
Molecular Weight 240.343
Flash Point 245.0±30.9 °C
Exact Mass 240.162643
PSA 38.05000
LogP 3.66
Appearance of Characters off-white to gray-pink
Vapour Pressure 0.0±1.0 mmHg at 25°C
Index of Refraction 1.630
Storage condition ?20°C
Water Solubility DMSO: >10mg/mL

 Safety Information

Symbol GHS05 GHS07 GHS09
GHS05, GHS07, GHS09
Signal Word Danger
Hazard Statements H302-H318-H400
Precautionary Statements P273-P280-P305 + P351 + P338
Hazard Codes Xn,N
Risk Phrases 22-41-50/53
Safety Phrases 26-39-60-61
RIDADR UN 3077 9 / PGIII

 Synonyms

4-Methyl-N-(3-phenylpropyl)-1,2-benzenediamine
IN1201
JSH-23
FD5025
1,2-Benzenediamine, 4-methyl-N-(3-phenylpropyl)-
NF-kappaB Activation Inhibitor II,JSH-23
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