Influenza Hemagglutinin (HA) Peptide

Modify Date: 2024-01-04 15:56:28

Influenza Hemagglutinin (HA) Peptide structure	Common Name	Influenza Hemagglutinin (HA) Peptide
	CAS Number	92000-76-5	Molecular Weight	1102.15000
	Density	1.414±0.06 g/cm3 (20ºC 760 Torr)	Boiling Point	N/A
	Molecular Formula	C₅₃H₆₇N₉O₁₇	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	N/A

Use of Influenza Hemagglutinin (HA) Peptide

HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). It is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.

Name	ha peptide
Synonym	More Synonyms

Description	HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). It is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Inflammation/Immunology Peptides
In Vitro	HA Peptide is a highly immunoreactive tag generally used for the separation of tagged proteins from cell culture supernatants and cell lysate under neutral pH conditions and thus are handy tools for coimmunoprecipitation but are also easily detected via western blot. HA Peptide is small and thus unlikely to interfere with the bioactivity and function of the fusion partner proteins. HA Peptide comes from human influenza hemagglutinin (HA) corresponding to amino acids 98–106 and is a strong immunoreactive epitope making it popular to isolate, purify, detect, and track the protein of interest. The recombinant HA-tagged proteins can be separated by highly specific anti-HA monoclonal antibody that is covalently immobilized on resin. The HA-tagged proteins can be eluted by mild elution approach with HA epitope at 1 mg/mL in TBS. On the other hand, three chemical elution options are available: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH[1]. The nucleotide sequences encoding an N-terminal HA Peptide in the mammalian expression vectors is an essential element for the T7 promoter-driven expression in E. coli even without trans-acting T7 RNAP[2]. Research results suggest that HA Peptide is cleaved by caspase 3/7, and HA Peptide cleavage results in a total loss of immunoreactivity. Observations indicate that the use of HA to tag proteins and constructs to study cell death–related and apoptotic mechanisms can result in serious artifacts[3].
References	[1]. Zhao X, et al. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093. [2]. Moon JM, et al. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6. [3]. Schembri L, et al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8.

Density	1.414±0.06 g/cm3 (20ºC 760 Torr)
Molecular Formula	C₅₃H₆₇N₉O₁₇
Molecular Weight	1102.15000
Exact Mass	1101.47000
PSA	413.83000
LogP	1.68080
Storage condition	-20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi
RIDADR	NONH for all modes of transport
WGK Germany	3

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TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA

HA1 fragment 98-106

HA1 FRAGMENT 99-107

INFLUENZA HEMAGGLUTININ (HA) PEPTIDE

HA TAG PEPTIDE

H2N-YPYDVPDYA-OH

YPYDVPDYA

H-TYR-PRO-TYR-ASP-VAL-PRO-ASP-TYR-ALA-OH