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刚果红

刚果红用途

Congo red 是一种偶氮染料。Congo red 结合已被广泛用于组织切片中淀粉样蛋白的测定。

刚果红名称

[ CAS 号 ]:
573-58-0

[ 中文名 ]:
刚果红

[ 英文名 ]:
Congo Red

[中文别名 ]:

[英文别名 ]:

DIRECT RED 28
Solucongo
Hemorrhagyl
Congo red
Haemomedical
EINECS 209-358-4
Congazone sodium
MFCD00004028
Haemonorm
Benzo Congo Red

刚果红生物活性

[ 描述 ]:

Congo red 是一种偶氮染料。Congo red 结合已被广泛用于组织切片中淀粉样蛋白的测定。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

研究领域 >> 其他

[体外研究]

刚果红组织化学染色可作为一种简单的筛选工具,用于研究突变细胞中的聚集体是否具有错误折叠的β-折叠片状二级结构。刚果红组织化学染料具有与交叉的β-褶皱片结构特异性结合的能力。野生型HSPB1应通过以非天然构象结合蛋白质来维持蛋白质稳态,从而防止底物聚集。然而,T139M突变体在该功能中失败并导致错误折叠的蛋白质的累积,其被刚果红靶向以插入β-折叠的片状结构之间。刚果红组织化学染色可以作为一个简单的工具,以研究突变细胞中的聚集体是否具有错误折叠的β-折叠片二级结构[1]。

[细胞实验]

由于其大的细胞质体积，选择HeLa细胞用于这些研究。用突变体或野生型HSPB1构建体转染的细胞在盖玻片上生长24小时，然后用刚果红染色以确定聚集体是否显示淀粉样蛋白形成特性。简而言之，首先用10％福尔马林固定细胞10分钟，并用1％刚果红染色5分钟，然后用0.01％氢氧化钾在50％乙醇中脱色。然后将盖玻片通过分级乙醇浓度进行脱水并安装在封固剂中，并在罗丹明过滤器下通过荧光显微镜检查[1]。

[参考文献]

[1]. Amornvit J, et al. A novel p.T139M mutation in HSPB1 highlighting the phenotypic spectrum in a family. Brain Behav. 2017 Jul 21;7(8):e00774.

[相关活性小分子]

刚果红物理化学性质

[ 密度 ]:
0.995 g/mL at 25 °C

[ 熔点 ]:
>360 °C(lit.)

[ 分子式 ]:
C₃₂H₂₂N₆Na₂O₆S₂

[ 分子量 ]:
696.663

[ 精确质量 ]:
696.083740

[ PSA ]:
232.64000

[ LogP ]:
10.78740

[ 外观性状 ]:
棕色-红色粉末

[ 储存条件 ]:
Flammables area

[ 水溶解性 ]:
soluble

刚果红MSDS

详细MSDS(安全技术说明书)

刚果红毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: QK1400000

CHEMICAL NAME :: 1-Naphthalenesulfonic acid, 3,3'-(4,4'-biphenylenebis(azo))bis(4-amino-, disodium salt

CAS REGISTRY NUMBER :: 573-58-0

LAST UPDATED :: 199710

DATA ITEMS CITED :: 38

MOLECULAR FORMULA :: C32-H24-N6-O6-S2.2Na

MOLECULAR WEIGHT :: 698.72

WISWESSER LINE NOTATION :: L66J BZ ESWQ CNUNR DR DNUN- CL66J BZ ESWQ &-NA- 2

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: Standard Draize test

ROUTE OF EXPOSURE :: Administration into the eye

SPECIES OBSERVED :: Rodent - rabbit

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Human - man

DOSE/DURATION :: 143 mg/kg

TOXIC EFFECTS :: Vascular - other changes

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Human - man

DOSE/DURATION :: 1429 ug/kg

TOXIC EFFECTS :: Behavioral - convulsions or effect on seizure threshold Lungs, Thorax, or Respiration - dyspnea Blood - change in clotting factors

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 15200 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LCLo - Lowest published lethal concentration

ROUTE OF EXPOSURE :: Inhalation

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 50 gm/m3/1H

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 160 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - chronic pulmonary edema Blood - change in clotting factors

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 250 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Mammal - cat

DOSE/DURATION :: 100 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - chronic pulmonary edema Blood - change in clotting factors

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 6 gm/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Administration onto the skin

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 4 gm/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 230 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - chronic pulmonary edema Blood - change in clotting factors

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Bird - pigeon

DOSE/DURATION :: 120 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Lungs, Thorax, or Respiration - chronic pulmonary edema Blood - change in clotting factors

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 140 mg/kg

SEX/DURATION :: female 9 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 200 mg/kg

SEX/DURATION :: female 9 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - Central Nervous System Reproductive - Specific Developmental Abnormalities - eye/ear Reproductive - Specific Developmental Abnormalities - urogenital system

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 200 mg/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - other effects to embryo

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 140 mg/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 5 gm/kg

SEX/DURATION :: multigeneration

TOXIC EFFECTS :: Reproductive - Fertility - female fertility index (e.g. # females pregnant per # sperm positive females; # females pregnant per # females mated)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 5 gm/kg

SEX/DURATION :: female 8-12 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - urogenital system Reproductive - Effects on Newborn - germ cell effects (in offspring) Reproductive - Effects on Newborn - delayed effects

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 5 gm/kg

SEX/DURATION :: female 8-12 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight gain) Reproductive - Effects on Newborn - physical

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 2500 ug/kg

SEX/DURATION :: multigeneration

TOXIC EFFECTS :: Reproductive - Paternal Effects - spermatogenesis (incl. genetic material, sperm morphology, motility, and count) Reproductive - Paternal Effects - testes, epididymis, sperm duct

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

DOSE :: 5 gm/kg

SEX/DURATION :: female 8-12 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Newborn - other postnatal measures or effects

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 300 mg/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - urogenital system

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 1500 mg/kg

SEX/DURATION :: female 8-12 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Newborn - growth statistics (e.g.%, reduced weight gain) Reproductive - Effects on Newborn - physical

TYPE OF TEST :: DNA adduct

MUTATION DATA

TYPE OF TEST :: Unscheduled DNA synthesis

TEST SYSTEM :: Rodent - hamster Liver

DOSE/DURATION :: 10 umol/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984 *** REVIEWS *** TOXICOLOGY REVIEW ARVPAX Annual Review of Pharmacology. (Palo Alto, CA) V.1-15, 1961-75. For publisher information, see ARPTDI. Volume(issue)/page/year: 5,447,1965 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - M3158 No. of Facilities: 384 (estimated) No. of Industries: 8 No. of Occupations: 8 No. of Employees: 1523 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - M3158 No. of Facilities: 284 (estimated) No. of Industries: 3 No. of Occupations: 6 No. of Employees: 4271 (estimated) No. of Female Employees: 3442 (estimated)

刚果红安全信息

[ 符号 ]:

GHS08

[ 信号词 ]:
Danger

[ 危害声明 ]:
H350-H361d

[ 警示性声明 ]:
P201-P280-P308 + P313

[ 个人防护装备 ]:
Eyeshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges

[ 危害码 (欧洲) ]:
T:Toxic

[ 风险声明 (欧洲) ]:
R45;R63

[ 安全声明 (欧洲) ]:
S53-S45-S37/39-S26

[ 危险品运输编码 ]:
2811

[ WGK德国 ]:
3

[ RTECS号 ]:
QK1400000

[ 危险类别 ]:
6.1

刚果红上下游产品

刚果红上游产品

刚果红下游产品

刚果红制备

1.由联欢苯胺重氮化后，与1，4-氨基萘磺酸钠进行偶合，而后经盐析；过滤及干燥而制得。具体操作为：取48.4g联苯胺溶于300ml盐酸（含浓盐酸20ml）中，加冰冷至5℃以下，加30ml浓盐酸后，加入由14.4g亚硝酸钠配成的10%的水溶液。重氮化结束后，将重氮盐溶液慢慢加入150g对氨基萘磺酸钠和少量水配成的溶液中，放置半小时后，在充分搅拌下慢慢加入35g碳酸钠，使溶液呈碱性，加热至约80℃后，冷却，过滤，用饱和盐水洗涤，干燥即得成品。

工业制备原料消耗（kg/t）联苯胺（100%） 200 氨基萘磺酸钠（100%） 520 亚硝酸钠（工业） 160 盐酸（31%） 330 纯碱（工业） 340 精盐 2500 太古油 9 乙醇 60 乙酸钠 35 元明粉 50

2.将联苯胺溶于适量盐酸中，加热至80～90℃，使联苯胺完全溶解，将溶液冷至0～5℃，再加入相同量的盐酸。然后维持温度在5℃以下，搅拌下滴加10%的亚硝酸钠水溶液进行反应：

然后在充分搅拌下加入碳酸钠至溶液呈碱性，加热至80℃后冷却结晶，过滤，结晶用饱和盐水洗涤，干燥即可。

刚果红文献

SUMO1 promotes Aβ production via the modulation of autophagy.

Autophagy 11(1) , 100-12, (2015)

Autophagy is one of the main mechanisms in the pathophysiology of neurodegenerative disease. The accumulation of autophagic vacuoles (AVs) in affected neurons is responsible for amyloid-β (Aβ) product...

Swift adsorptive removal of Congo red from aqueous solution by K1.33Mn8O16 nanowires.

J. Nanosci. Nanotechnol. 13(8) , 5452-60, (2013)

A swift and efficient approach to converting organic dye effluents into fresh water could be of substantial benefit. In this study, we presented facile hydrothermal synthesis of K1.33Mn8O16 nanowires ...

Phosphorylation-independent regulation of the diguanylate cyclase WspR.

PLoS Biol. 6 , e67, (2008)

Environmental signals that trigger bacterial pathogenesis and biofilm formation are mediated by changes in the level of cyclic dimeric guanosine monophosphate (c-di-GMP), a unique eubacterial second m...

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刚果红