NCB-0846
Names
[ CAS No. ]:
1792999-26-8
[ Name ]:
NCB-0846
[Synonym ]:
4-[2-(3H-benzimidazol-5-ylamino)quinazolin-8-yl]oxycyclohexan-1-ol
Biological Activity
[Description]:
NCB-0846 is an orally available TNIK inhibitor with an IC50 of 21 nM.
[Related Catalog]:
[Target]
Wnt
TNIK:21 nM (IC50)
[In Vitro]
NCB-0846 has anti-Wnt activity. NCB-0846 binds to TNIK in an inactive conformation, and this binding mode seems to be essential for Wnt inhibition. NCB-0846 shows inhibitory activity against TNIK with an IC50 of 21 nM. NCB-0846 also inhibits FLT3, JAK3, PDGFRα, TRKA, CDK2/CycA2, and HGK. NCB-0846 induces faster migration of TCF4 phosphorylated by TNIK within a concentration range of 0.1-0.3 μM and completely inhibits the phosphorylation of TCF4 at a concentration of 3 μM. NCB-0846 inhibits HCT116 cell growth and shows much higher (-20-fold) inhibitory activity against colony formation by the same cells in soft agar[1].
[In Vivo]
NCB-0846 suppresses the growth of tumors established by inoculating HCT116 cells into immunodeficient mice. The expression of Wnt-target genes (AXIN2, MYC and CCND1) in xenografts is reduced following the administration of NCB-0846. NCB-0846 induces an increase in the sub-G1 cell population. Cleavage of poly (ADP-ribose) polymerase 1 indicates the induction of apoptosis[1].
[Animal admin]
Mice: NCB-0846 is suspended in DMSO/polyethylene glycol#400/30% 2-hydroxypropyl-β-cyclodextrin solution (10:45:45v/v). Five million HCT116 cells suspended in medium containing 25% Matrigel are inoculated into the subcutaneous tissues of 9-week-old female BALB/c nude mice. Mice are randomized according to tumour volume (9 mice per group). NCB-0846 was administered daily by oral gavage at 0 (vehicle alone), 40 or 80 mg/kg (body weight) BID (bis in die) for 14 days[1].
[References]
[Related Small Molecules]
ICG-001
|
Salinomycin
|
IWR-1
|
Wnt-C59
|
NG25
|
Berberine chloride hydrate
|
Pyrvinium pamoate
|
ETC-159
|
BML 284
|
IWP-2
|
Heparan Sulfate
|
GNE-495
|
KYA 1797K
|
FH535
|
SKL2001
Chemical & Physical Properties
[ Molecular Formula ]:
C21H21N5O2
[ Molecular Weight ]:
375.432
[ Exact Mass ]:
375.17
[ Storage condition ]:
-20℃