9-hydroxystearic acid

9-Hydroxyoctadecanoic acid (9-HSA) is an HDAC1 inhibitor that inhibits ∼66.4% HDAC1 enzymatic activity at 5 μM. 9-Hydroxyoctadecanoic acid shows anticancer activity[1].

Research Areas >> Cancer

Signaling Pathways >> Cell Cycle/DNA Damage >> HDAC

Signaling Pathways >> Epigenetics >> HDAC

HDAC1

9-Hydroxyoctadecanoic acid (9-HSA) 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。 9-Hydroxyoctadecanoic acid (9-HSA) (100 μM; 24 h) 抑制 HT29 细胞增殖，诱导 G0/G1 阻滞，并在转录和翻译水平上增加 p21WAF1 表达[2]。 Cell Proliferation Assay9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。 Cell Line: HT29 cell Concentration: 100 μM Incubation Time: 24 h Result: Resulted in a significant inhibition of cell proliferation. Cell Cycle Analysis9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。 Cell Line: HT29 cell Concentration: 100 μM Incubation Time: 24 h Result: Decreased S-phase activity by 50.2% compared with untreated controls, and the growth inhibition was associated with a strong arrest in G0/G1. Western Blot Analysis9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。 Cell Line: HT29 cell Concentration: 100 μM Incubation Time: 24 h Result: Increased the expression of p21WAF1. RT-PCR9-Hydroxyoctadecanoic acid 可以结合到人 HDAC1 蛋白三维模型的活性位点[1]。 Cell Line: HT29 cell Concentration: 100 μM Incubation Time: 24 h Result: Induced p21WAF1 tanscript.

[1]. Calonghi N, et al. Histone deacetylase 1: a target of 9-hydroxystearic acid in the inhibition of cell growth in human colon cancer. J Lipid Res. 2005 Aug;46(8):1596-603.  

[2]. Calonghi N, et al. 9-Hydroxystearic acid upregulates p21(WAF1) in HT29 cancer cells. Biochem Biophys Res Commun. 2004 Jan 30;314(1):138-42.