Bis-ANS dipotassium

Names

[ Name ]:
Bis-ANS dipotassium

[Synonym ]:
BIS-ANS
BIS-ANS DIPOTASSIUM SALT
4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid dipotassium salt
4,4'-DIANILINO-1,1'-BINAPHTHYL-5,5'-DISULFONIC ACID,POTASSIUM SALT
potassium 8,8'-dianilino-5,5'-binaphthalene-1,1'-disulfonate
4,4'-dianilino-1,1'-binaphthyl-5,5'-disulfonic acid,dipot
8-Anilino-1-naphthalenesulfonic Acid Di
4,4'-dianilino-1,1'-binaphthyl-5,5'-di-sulfonic A
4,4'-DIANILINO-1,1'-BINAPHTHYL-5,5'-DISU LFONIC ACI

Biological Activity

[Description]:

Bis-ANS dipotassium is a fluorescent probe of hydrophobic protein. Bis-ANS binds to tubulin with a Kd of 2 μM[1]. Bis-ANS dipotassium is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS). Bis-ANS dipotassium promotes LLPS at low concentrations but suppresses LLPS at high concentrations[2].

[Related Catalog]:

Signaling Pathways >> Cell Cycle/DNA Damage >> Microtubule/Tubulin

Signaling Pathways >> Cytoskeleton >> Microtubule/Tubulin

Research Areas >> Neurological Disease

[In Vitro]

Bis-ANS is a fluorescent molecule that has been used to probe exposed hydrophobic patches in proteins and to monitor the formation of protein aggregates. Bis-ANS is a bivalent naphthalene sulfonate with aniline moieties. Bis-ANS modulates the liquid-liquid phase separation of TDP-43 low complexity domain (LCD)[2].

[References]

[1]. P Horowitz, et al. Bis(1,8-anilinonaphthalenesulfonate). A novel and potent inhibitor of microtubule assembly. J Biol Chem. 1984 Dec 10;259(23):14647-50.

[2]. W. Michael Babinchak, et al. Small molecules as potent biphasic modulators of protein liquid-liquid phase separation. Nature Communications volume 11, Article number: 5574 (2020)

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₂H₂₂K₂N₂O₆S₂

[ Molecular Weight ]:
672.85300

[ Exact Mass ]:
672.01900

[ PSA ]:
155.22000

[ LogP ]:
9.26300

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H302-H312-H315-H319-H332-H335

[ Precautionary Statements ]:
P261-P280-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xn

[ Risk Phrases ]:
R20/21/22;R36/37/38

[ Safety Phrases ]:
S26-S36

[ RIDADR ]:
NONH for all modes of transport

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Articles

Interaction of ATP with a small heat shock protein from Mycobacterium leprae: effect on its structure and function.

PLoS Negl. Trop. Dis. 9(3) , e0003661, (2015)

Adenosine-5'-triphosphate (ATP) is an important phosphate metabolite abundantly found in Mycobacterium leprae bacilli. This pathogen does not derive ATP from its host but has its own mechanism for the...

Bis(8-anilinonaphthalene-1-sulfonate) as a probe for tubulin decay.

Biochemistry 25 , 739, (1986)

The fluorescent apolar probe bis(8-anilinonaphthalene-1-sulfonate) (Bis-ANS) has been used to detect structural correlates of the well-known but poorly understood decay of tubulin function, by which t...

Cooperative effects in binding by bovine serum albumin. I. The binding of 1-anilino-8-naphthalenesulfonate. Fluorimetric titrations.

Biochemistry 5 , 1893, (1966)