ddATP trisodium

ddATP (2',3'-Dideoxyadenosine 5'-triphosphate) trisodium, an active metabolite of 2',3'-dideoxyinosine, is a chain-elongating inhibitor of DNA polymerase. ddATP trisodium can be used for Sanger method for DNA sequencing and research of virus infection[1][2][4].

Signaling Pathways >> Anti-infection >> HIV

Research Areas >> Infection

Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis

ddATP (0.05-50 μM, 30 min) trisodium inhibits adenovirus DNA synthesis in isolated nuclei from adenovirus-infected KB cells[1]. ddATP (100 μM, 5 h) trisodium reduces the number of γH2A.X foci in the DSB (DNA double-strand breaks) oocytes[3]. ddATP trisodium inhibits HIV replication by inhibiting HIV reverse transcriptase[5]. Immunofluorescence[3] Cell Line: DSB oocytes Concentration: 100 μM Incubation Time: 5 h Result: Suppressed the mtDNA replication without fully suppressed the nuclear DNA replication.

[1]. van der Vliet PC, et al. Role of DNA polymerase gamma in adenovirus DNA replication. Mechanism of inhibition by 2',3'-dideoxynucleoside 5'-triphosphates. Biochemistry. 1981 Apr 28;20(9):2628-32.

[2]. Xu Lan, et al. Simultaneous determination of 2 ,3 -dideoxyinosine and the active metabolite, 2 ,3 -dideoxyadenosine-5 -triphosphate in human peripheral-blood mononuclear cell by HPLC–MS/MS and the application to cell pharmacokinetics. J Chromatogr B Analyt Technol Biomed Life Sci. 2015 Oct 1;1002:337-42.

[3]. Jun-Yu Ma, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054.

[4]. C M Perry, et al. Didanosine. An update on its antiviral activity, pharmacokinetic properties and therapeutic efficacy in the management of HIV disease. Drugs. 1996 Dec;52(6):928-62.

[5]. Hao Z, et al. Factors determining the activity of 2',3'-dideoxynucleosides in suppressing human immunodeficiency virus in vitro. Mol Pharmacol. 1988 Oct;34(4):431-5.