(S)-2-chloro-5,6,7,8,9,10-hexahydrocyclohepta[b]indole-6-carboxamide
Names
[ CAS No. ]:
848193-72-6
[ Name ]:
(S)-2-chloro-5,6,7,8,9,10-hexahydrocyclohepta[b]indole-6-carboxamide
[Synonym ]:
chic-35
Biological Activity
[Description]:
CHIC35, an analog of EX-527, is a potent and selective inhibitor of SIRT1 (IC50=0.124 µM). CHIC35 shows potential selective inhibition against SIRT1 over SIRT2 (IC50=2.8 µM) or SIRT3 (IC50>100 µM)[1]. CHIC35 has anti-inflammatory effects and can be used for CHARGE syndrome research[1][2].
[Related Catalog]:
[Target]
SIRT1:0.124 μM (IC50)
SIRT2:2.8 μM (IC50)
SIRT3:>100 μM (IC50)
[In Vitro]
CHIC-35 (0.5 μM; 16 hours) increases acetylation of histone H4 in BMDMs similar to Cambinol (200 μM)[1]. CHIC-35 (5 μM; 72 hours) exhibits no significant difference in the survival of embryos at early stages[2]. Zebrafish embryos are microinjected with 2.4 ng of chd7 MO to develop to different stages of development. chd7 morphant embryos are treated with CHIC-35 from 8hpf to 24hpf. CHIC-35 (5 μM) is removed at 24hpf and embryos are incubated in fresh egg water until 4dpf. The chd7 morphant larvae has a severely reduced and disrupted pattern of cartilage elements in comparison to the control, CHIC-35 shows partial recovery in craniofacial cartilage elements[2]. At 4dpf, zebrafish embryos show a well-formed lower jaw in controls, while chd7 morphants exhibits reduced lower jaw. Treatment with CHIC-35 (5 μM) rescues the expression of sox9a inchd7 morphants[2]. Nearly 30% of chd7 morphant embryos (24hpf to 72hpf) shows a near complete loss of isl2a expression in the cranial region compared to 10% of the wildtype controls. CHIC-35 reduces this to 7.5% significantly. However, CHIC-35 shows no discernible effect on the enteric neurons marked by Tg[2].
[References]
Chemical & Physical Properties
[ Molecular Formula ]:
C14H15ClN2O
[ Molecular Weight ]:
262.73500
[ Exact Mass ]:
262.08700
[ PSA ]:
59.87000
[ LogP ]:
4.26630
[ Storage condition ]:
2-8°C
Safety Information
[ Symbol ]:
GHS06
[ Signal Word ]:
Danger
[ Hazard Statements ]:
H301-H315-H319-H335
[ Precautionary Statements ]:
P261-P301 + P310-P305 + P351 + P338
[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Faceshields;Gloves
[ RIDADR ]:
UN 2811 6.1 / PGIII