T7 RNA polymerase

Names

[ Name ]:
T7 RNA polymerase

[Synonym ]:
EINECS 232-756-4

Biological Activity

[Description]:

T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter[1][2].

[Related Catalog]:

Signaling Pathways >> Cell Cycle/DNA Damage >> DNA/RNA Synthesis

Research Areas >> Others

[References]

[1]. Dousis A, et al. An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts. Nat Biotechnol. 2023 Apr;41(4):560-568.

[2]. Borkotoky S, et al. The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt A):49-56.

Chemical & Physical Properties

[ Storage condition ]:
−20°C

Safety Information

[ Hazard Codes ]:
Xi

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

Sci. Signal. 2 , ra46, (2009)

Protein phosphorylation events during T cell receptor (TCR) signaling control the formation of complexes among proteins proximal to the TCR, the activation of kinase cascades, and the activation of tr...

Initial characterization of the human central proteome.

BMC Syst. Biol. 5 , 17, (2011)

On the basis of large proteomics datasets measured from seven human cell lines we consider their intersection as an approximation of the human central proteome, which is the set of proteins ubiquitous...

The DNA sequence and biology of human chromosome 19.

Nature 428(6982) , 529-35, (2004)

Chromosome 19 has the highest gene density of all human chromosomes, more than double the genome-wide average. The large clustered gene families, corresponding high G + C content, CpG islands and dens...