Name | Leishman Stain |
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Synonyms |
EINECS 235-732-1
MFCD00131498 |
Description | Leishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens)[1]. |
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Related Catalog | |
In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Prepare stock solution of Sorensen’s buffer: Solution A. Na2HPO4•2H2O (9.5 g/L); Solution B. KH2PO4 (9.1 g/L). 2. Cover the smear with Leishman stain for 2 min. 3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min. 4. Wash off with running water and dry the smear (5 min). |
References |
Molecular Formula | n.a. |
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Appearance | Powder |
Storage condition | Store at +15°C to +30°C. |
Water Solubility | methanol: soluble1mg/mL | Soluble in water (4 mg/ml), methanol (1 mg/ml), ethanol (4 mg/ml), and methyl cellosolve (20 mg/ml). |
Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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Hazard Codes | Xi |
Risk Phrases | 36/38-36/37/38 |
Safety Phrases | S22;S24/25 |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |