Shanghai Nianxing Industrial Co., Ltd
China
Product Name: C527
Price:
Purity: 98.0%
Stocking Period: 10 Day
Contact: Yang

DC Chemicals Limited
China
Product Name: C527
Price: $Inquiry/100mg $Inquiry/250mg $Inquiry/500mg
Purity: 98.0%
Stocking Period: 3 Day
Contact: Tony Cao

Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: 2-(4-Fluorophenyl)naphtho[2,3-d]oxazole-4,9-dione
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
Stocking Period: Inquiry
Contact: Ms Wang

192718-06-2

192718-06-2 structure

192718-06-2 structure

Name: C527
Chemical Name: C527
CAS Number: 192718-06-2
Molecular Formula: C₁₇H₈FNO₃
Molecular Weight: 293.249
Catalog: Signaling Pathways Cell Cycle/DNA Damage Deubiquitinase
Create Date: 2018-06-27 22:28:11
Modify Date: 2024-01-09 18:47:02
C527 is a is a pan DUB enzyme inhibitor, with a high potency for the USP1/UAF1 complex (IC50=0.88 μM).

Name	C527
Synonyms	2-(4-Fluorophenyl)naphtho[2,3-d][1,3]oxazole-4,9-dione MFCD01033540 Naphth[2,3-d]oxazole-4,9-dione, 2-(4-fluorophenyl)-

Description	C527 is a is a pan DUB enzyme inhibitor, with a high potency for the USP1/UAF1 complex (IC50=0.88 μM).
Related Catalog	Signaling Pathways >> Cell Cycle/DNA Damage >> Deubiquitinase Research Areas >> Cancer
Target	0.88 μM (USP1)[1]
In Vitro	Pretreatment of USP1/UAF1 with C527 resulted in inhibition of its enzyme activity with an IC50 of 0.88±0.03 μM. C527 inhibits the DUB activity of the USP12/USP46 complex and other DUB enzymes in vitro. However, the IC50 of C527 for these DUB enzymes was higher in comparison with USP1/UAF1 complex. C527 has considerably less inhibitory effect on UCH-L1 and UCH-L3, a different subclass of DUB enzymes. C527 treatments causes an increase in the levels of Ub-FANCD2 and Ub-FANCI. Pretreatment of cells with the C527 causes an enhancement in the cytoxicity of mitomycin C and camptothecin. C527 treatments lead to an increase in ubiquitinated forms of FANCD2 and FANCI, cause a decrease in homologous recombination activity, and sensitize cells to DNA damaging agents[1].
Cell Assay	HeLa cells are treated with DMSO or C527 (0.5, 1, 5 μM) in appropriate medium for 24 to 72 hours. The viable cell counts are determined using Trypan Blue staining, CellTiter-Glo reagent or MTT assay[1].
References	[1]. Mistry H, et al. Small-molecule inhibitors of USP1 target ID1 degradation in leukemic cells. Mol Cancer Ther. 2013 Dec;12(12):2651-62.

Density	1.4±0.1 g/cm3
Boiling Point	488.6±47.0 °C at 760 mmHg
Molecular Formula	C₁₇H₈FNO₃
Molecular Weight	293.249
Flash Point	249.3±29.3 °C
Exact Mass	293.048828
LogP	4.82
Vapour Pressure	0.0±1.2 mmHg at 25°C
Index of Refraction	1.639
Storage condition	2-8℃

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302
RIDADR	NONH for all modes of transport