2260791-13-5
2260791-13-5 structure
- Name: OSMI-3
- Chemical Name: OSMI-3
- CAS Number: 2260791-13-5
- Molecular Formula: C32H35N3O9S2
- Molecular Weight: 669.77
- Catalog: Research Areas Metabolic Disease
- Create Date: 2020-04-10 02:11:23
- Modify Date: 2024-01-16 10:37:14
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OSMI-3 (Compound 2b) is a potent, long-lasting, and cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-3 increases detained intron splicing in cells[1].
| Name | OSMI-3 |
|---|
| Description | OSMI-3 (Compound 2b) is a potent, long-lasting, and cell-permeable O-linked N-acetylglucosamine transferase (OGT) inhibitor. Cells contain a large nuclear pool of partially spliced OGT transcript, and OSMI-3 increases detained intron splicing in cells[1]. |
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| Related Catalog | |
| Target |
O-linked N-acetylglucosamine transferase (OGT)[1] |
| In Vitro | OSMI-3 (Compound 2b; 20-50 μM; 4-24 hours; HCT116 cells) treatment significantly reduces O-GlcNAc levels, and has more sustained cellular effects than OSMI-2[1]. A decrease in HCF-1 cleavage products and the appearance of uncleaved HCF-1 in cells treated with OSMI-3 (Compound 2b) is observed. Because OGT knockdown is known to decrease cell proliferation, the effects of OSMI-3 on cell growth in culture over 96h is also monitored. Although there is no evidence of apoptosis, the reduced growth of cells over time is observed, consistent with the knockdown results[1]. Western Blot Analysis[1] Cell Line: HCT116 cells Concentration: 20 μM, 40 μM, 50 μM Incubation Time: 4 hours, 24 hours Result: Reduced O-GlcNAc levels. |
| References |
| Molecular Formula | C32H35N3O9S2 |
|---|---|
| Molecular Weight | 669.77 |