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193149-74-5

193149-74-5 structure
193149-74-5 structure
  • Name: WST-8
  • Chemical Name: sodium,4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
  • CAS Number: 193149-74-5
  • Molecular Formula: C20H14N6NaO11S2
  • Molecular Weight: 601.48
  • Catalog: Biochemical Assay Reagents
  • Create Date: 2018-02-20 08:00:00
  • Modify Date: 2024-01-02 20:39:21
  • WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

Name sodium,4-[2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)tetrazol-2-ium-5-yl]benzene-1,3-disulfonate
Synonyms Sodium 4-[3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl]-1,3-benzenedisulfonate
wst-8
2H-Tetrazolium, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-, inner salt, sodium salt (1:1)
Description WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.
Related Catalog
In Vitro The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].
References

[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.

Molecular Formula C20H14N6NaO11S2
Molecular Weight 601.48
PSA 266.62000
LogP 4.05220
Storage condition 2~8°C
Hazard Codes C