In Vitro |
ASR-490 (0-1.6 µM; 24 h, 48 h) reduces the viability of HCT116 and SW620 cells in 24h (IC50 =750 nM in HCT116 cells, IC50 =1.2 µM in SW620 cells) and 48h (IC50 =600 nM in HCT116 cells, IC50 =850 nM in SW620 cells)[1].ASR-490 (750 nM in HCT116 cells and 1.2 µM in SW620 cells, 24 h) shows apoptotic cell death and upregulation of the proapoptotic markers Bax and cleaved PARP Expression; inhibits the capability of colorectal cancer cells[1].ASR-490 (HCT116 cells; 750 nM in 24 h, 600 nM in 48 h) overcomes Notch1 overexpression and inhibits the growth of HCT/Notch1 transfectants[1]. Cell Viability Assay[1] Cell Line: HCT116, SW620 cells Concentration: 0-1.6 µM Incubation Time: 24 h, 48 h Result: Reduced the viability of HCT116 and SW620 cells in 24h (IC50 =750 nM in HCT116 cells, IC50 =1.2 µM in SW620 cells) and 48h (IC50 =600 nM in HCT116 cells, IC50 =850 nM in SW620 cells). Apoptosis Analysis[1] Cell Line: HCT116, SW620 cells Concentration: 750 nM in HCT116 cells and 1.2 µM in SW620 cells Incubation Time: 24 h Result: Showed apoptotic cell death and upregulation the proapoptotic markers Bax and cleaved PARPExpression, inhibited the capability of colorectal cancer cells. Cell Proliferation Assay[1] Cell Line: HCT116 cells Concentration: 750 nM in 24 h, 600 nM in 48 h Incubation Time: 24 h, 48 h Result: Overcome Notch1 overexpression and inhibited the growth of HCT/Notch1 transfectants.
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