216571-99-2
216571-99-2 structure
- Name: BODIPY FL Thapsigargin
- Chemical Name: BODIPY FL Thapsigargin
- CAS Number: 216571-99-2
- Molecular Formula: C44H57B3F2N2O122-
- Molecular Weight: 876.36
- Catalog: Research Areas Others
- Create Date: 2022-10-15 17:16:01
- Modify Date: 2024-04-06 17:28:17
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BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells[1][2].
| Name | BODIPY FL Thapsigargin |
|---|
| Description | BODIPY FL Thapsigargin is a potent green fluorescent dye. BODIPY FL Thapsigargin inhibits intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum. BODIPY FL Thapsigargin used for investigation of thapsigargin binding sites in live cells[1][2]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). BODIPY FL Thapsigargin[1]: 1. Purified parasites are fixed with 1% formaldehyde in PBS (pH 8) for 1 h at room temperature and allows to adhere to poly-L-lysine-coated glass slides for 20 min Parasites are then permeated with 0.1% Triton X-100 for 5 min and blocked with 3% bovine serum albumin in PBS for 1 h. 2. Parasites were incubated at room temperature with a 1:100 dilution of the primary polyclonal antibody H-300 for 1 h, followed by a 1:100 dilution of Texa Red-coupled goat anti-rabbit IgG secondary antibody, and with 0.25 µM BODIPY FL TG for 5 min. 3. Analyze sample on a flow cytometer, fluorescence microscopy, or fluorescence microplate reader. |
| References |
| Molecular Formula | C44H57B3F2N2O122- |
|---|---|
| Molecular Weight | 876.36 |