Name | Cyanine3 maleimide tetrafluoroborate |
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Description | Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling[1][2]. |
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Related Catalog | |
In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Protein:1.The protein concentration was adjusted to 1 mg/mL for 100 μL.2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL).3. Incubated overnight at 4 °C in the dark.4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate.5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively. |
References |
Molecular Formula | C36H43BF4N4O32- |
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Molecular Weight | 666.56 |