1239-45-8

1239-45-8 structure

Name: Ethidium bromide
Chemical Name: ethidium bromide
CAS Number: 1239-45-8
Molecular Formula: C₂₁H₂₀BrN₃
Molecular Weight: 394.308
Catalog: Biochemical Biochemical reagent Biological dye
Create Date: 2018-08-30 15:24:22
Modify Date: 2024-01-02 12:30:30
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Name	ethidium bromide
Synonyms	3,8-Diamino-5 MFCD00011724 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide,EtBr,Homidium bromide Phenanthridinium, 3,8-diamino-5-ethyl-6-phenyl-, bromide (1:1) Homidium bromide 3,8-Diamino-5-ethyl-6-phenylphenanthridin-5-ium bromide 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide EINECS 214-984-6 5-ethyl-6-phenylphenanthridin-5-ium-3,8-diamine,bromide EthidiuM BroMide

Description	Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The preparation of agarose gel: 1． Agarose gels are commonly used in concentrations of 0.5% to 2.5% depending on the size of bands needed to be separated. 2． Mix the agarose powder with 1X TAE/TBE. 3． Microwave for 1-3 min until the agarose is completely dissolved (Caution: not overboil). 4． Make the solution cool down before solidification. 5． Add ethidium bromide (EtBr) to a final concentration of approximately 0.2-0.5 μg/mL (Stocks are generally 10 mg/ml, and require 5 µL stock/100 mL gel). 6． Ethidium bromide binds to the DNA and you could visualize the DNA under ultraviolet (UV) light. CAUTION: EtBr is a known mutagen. Please pay attention to strengthening protection.
References	[1]. P Stevenson, et al. Comparison of isometamidium chloride and homidium bromide as prophylactic drugs for trypanosomiasis in cattle at Nguruman, Kenya. Acta Trop. 1995 May;59(2):77-84. [2]. Sabnis, Ram Wasudeo (2010). Handbook of Biological Dyes and Stains: Synthesis and Industrial Application. Hoboken, NJ: Wiley. ISBN 978-0-470-40753-0.

Melting Point	260-262 °C (dec.)(lit.)
Molecular Formula	C₂₁H₂₀BrN₃
Molecular Weight	394.308
Flash Point	>100°C
Exact Mass	393.084045
PSA	55.92000
LogP	2.29820
Index of Refraction	1.4575 (20ºC)
Storage condition	2-8°C
Stability	Stable. Incompatible with strong oxidizing agents.
Water Solubility	40 g/L (25 ºC)

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

REFERENCE :: 47JMAE "Cytogenetic Assays of Environmental Mutagens," Hsu, T.C., ed., Totowa, NJ, Allanheld, Osmun & Co., 1982 Volume(issue)/page/year: -,137,1982 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - A1168 No. of Facilities: 17 (estimated) No. of Industries: 1 No. of Occupations: 1 No. of Employees: 34 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - A1168 No. of Facilities: 7 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 21 (estimated)

Symbol	GHS06, GHS08
Signal Word	Danger
Hazard Statements	H302-H330-H341
Precautionary Statements	P260-P281-P284-P310
Personal Protective Equipment	Eyeshields;Faceshields;full-face particle respirator type N100 (US);Gloves;respirator cartridge type N100 (US);type P1 (EN143) respirator filter;type P3 (EN 143) respirator cartridges
Hazard Codes	T+:Verytoxic;
Risk Phrases	R21/22;R26;R36/37/38;R68
Safety Phrases	S36/37-S45-S36/37/39-S28A-S26-S22
RIDADR	UN 2811 6.1/PG 1
WGK Germany	3
RTECS	SF7950000
Packaging Group	I
Hazard Class	6.1
HS Code	29339990

HS Code	2933990090
Summary	2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%