2503-26-6
2503-26-6 structure
- Name: Destruxin B
- Chemical Name: .β.-Alanine, N-[N-[N-[N-[1-(D-2-hydroxy-4-methyl-1-oxopentyl)-L-prolyl]-L-isoleucyl]-N-methyl-L-valyl]-N-methyl-L-alanyl]-, .ρ.-lactone
- CAS Number: 2503-26-6
- Molecular Formula: C32H55N5O7
- Molecular Weight: 593.75500
- Catalog: Signaling Pathways Apoptosis Apoptosis
- Create Date: 2016-09-06 19:24:15
- Modify Date: 2024-01-09 17:00:43
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Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells[1]. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo[2].
| Name | .β.-Alanine, N-[N-[N-[N-[1-(D-2-hydroxy-4-methyl-1-oxopentyl)-L-prolyl]-L-isoleucyl]-N-methyl-L-valyl]-N-methyl-L-alanyl]-, .ρ.-lactone |
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| Synonyms | destruxin B |
| Description | Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. Destruxin B induces apoptosis via a Bcl-2 Family-dependent mitochondrial pathway in human nonsmall cell lung cancer cells[1]. Destruxin B significantly activates caspase-3 and reduces tumor cell proliferation through caspase-mediated apoptosis, not only in vitro but also in vivo[2]. |
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| Related Catalog | |
| Target |
Bcl-2 Caspase-3 |
| In Vitro | Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC50s of 4.1 μM and 4.9 μM, respectively[1]. Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells[1]. Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners[2]. Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner [1]. Cell Proliferation Assay[1] Cell Line: H1299 cells Concentration: 1, 5, 10, 20, 30 μM Incubation Time: 48 hours Result: Inhibited H1299 cells proliferation of H1299 cells with an IC50 of 4.1 μM. Apoptosis Analysis[1] Cell Line: Lung adenocarcinoma A549 cells Concentration: 1, 5, 10, 20, 30 μM Incubation Time: 48 hours Result: Induces caspase-dependent Lung adenocarcinoma A549 cells death. Cell Viability Assay[2] Cell Line: HT-29 human colorectal cancer cells Concentration: 1.25, 2.50, 5.00, 10.00 and 20.00 μM Incubation Time: 72 hours Result: The IC50s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively. Western Blot Analysis[1] Cell Line: A549 cells Concentration: 10 or 20 μM Incubation Time: 12 and 24 hours Result: Regulates Mcl-1 and PUMA. |
| In Vivo | Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners[2]. Animal Model: Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival[2] Dosage: Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg) Administration: Injection; daily; for 6 weeks Result: Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively. |
| References |
| Density | 1.17g/cm3 |
|---|---|
| Boiling Point | 875ºC at 760mmHg |
| Molecular Formula | C32H55N5O7 |
| Molecular Weight | 593.75500 |
| Flash Point | 483ºC |
| Exact Mass | 593.37900 |
| PSA | 145.43000 |
| LogP | 1.78730 |
| Vapour Pressure | 4.15E-31mmHg at 25°C |
| Index of Refraction | 1.535 |
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATAACUTE TOXICITY DATA
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| Precursor 5 | |
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| DownStream 0 | |
