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81-93-6

81-93-6 structure
81-93-6 structure
  • Name: Phenosafranine
  • Chemical Name: safranin O
  • CAS Number: 81-93-6
  • Molecular Formula: C18H15ClN4
  • Molecular Weight: 322.792
  • Catalog: Biochemical Biochemical reagent Biological dye
  • Create Date: 2018-09-01 14:04:00
  • Modify Date: 2024-01-04 19:43:05
  • Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on[1][2][3].

Name safranin O
Synonyms Phenazinium, 3,7-diamino-5-phenyl-, chloride (1:1)
Phenosafranin
3,7-diamino-5-phenyl phenazinium chloride
MFCD00036335
Phenosafranine
3,7-Diamino-5-phenylphenazin-5-ium chloride
EINECS 201-387-0
Phenosofranine
Safrinin B Extra
Phenosafrinine
Phenosofrinine
Schultz No. 958
Safranin B Extra
10-phenylphenazin-10-ium-2,8-diamine,chloride
Phenosafranine,chloride
Description Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on[1][2][3].
Related Catalog
References

[1]. Widholm JM. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 1972 Jul;47(4):189-94.

[2]. Liu, W., et al. Characterization of Phenosafranine–Hemoglobin Interactions in Aqueous Solution. J Solution Chem 40, 231–246 (2011).

[3]. Pradhan AB, et al. An overview on the interaction of phenazinium dye phenosafranine to RNA triple and double helices. Int J Biol Macromol. 2016 May;86:345-51.

Density 1.1738 (rough estimate)
Boiling Point 478.49°C (rough estimate)
Melting Point 300ºC
Molecular Formula C18H15ClN4
Molecular Weight 322.792
Exact Mass 322.098511
PSA 68.81000
LogP 0.99550
Index of Refraction 1.6110 (estimate)

CHEMICAL IDENTIFICATION

RTECS NUMBER :
SG1630000
CHEMICAL NAME :
Phenazinium, 3,7-diamino-5-phenyl-, chloride
CAS REGISTRY NUMBER :
81-93-6
LAST UPDATED :
199612
DATA ITEMS CITED :
5
MOLECULAR FORMULA :
C18-H15-N4.Cl
MOLECULAR WEIGHT :
322.82
WISWESSER LINE NOTATION :
T C666 BK INJ BR& EZ MZ &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intraperitoneal
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
112 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value
TYPE OF TEST :
LD50 - Lethal dose, 50 percent kill
ROUTE OF EXPOSURE :
Intravenous
SPECIES OBSERVED :
Rodent - mouse
DOSE/DURATION :
18 mg/kg
TOXIC EFFECTS :
Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TEST SYSTEM :
Insect - Drosophila melanogaster
DOSE/DURATION :
1000 ppm
REFERENCE :
AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 87,295,1953
Symbol GHS07
GHS07
Signal Word Warning
Hazard Statements H315-H319-H335
Precautionary Statements P261-P305 + P351 + P338
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xi: Irritant;
Risk Phrases R36/37/38
Safety Phrases 26-36
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS SG1630000