Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: Dihydrorhodamine 123
Price: ￥938.0/5mg ￥418.0/1mg
Purity: 95.0%
Stocking Period: Inquiry
Contact: Liu jia

109244-58-8

109244-58-8 structure

109244-58-8 structure

Name: Dihydrorhodamine 123
Chemical Name: Dihydrorhodamine 123
CAS Number: 109244-58-8
Molecular Formula: C₂₁H₁₈N₂O₃
Molecular Weight: 346.379
Catalog: Dye Reagents
Create Date: 2018-03-04 08:00:00
Modify Date: 2024-01-11 16:51:02
Dihydrorhodamine 123 is a fluorescent probe (λex=488 nm, λem=525 nm).

Name	Dihydrorhodamine 123
Synonyms	MFCD00285712 Benzoic acid, 2-(3,6-diamino-9H-xanthen-9-yl)-, methyl ester Methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate

Description	Dihydrorhodamine 123 is a fluorescent probe (λex=488 nm, λem=525 nm).
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Others Dye Reagents
In Vitro	In the presence of 10 μM Dihydrorhodamine 123 (DHR) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1].
Cell Assay	The HL60 cells are incubated at 6×106 cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O2- is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×105 cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na+ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester[1].
References	[1]. Lydia M. Henderson et al. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.

Density	1.3±0.1 g/cm3
Boiling Point	526.9±50.0 °C at 760 mmHg
Molecular Formula	C₂₁H₁₈N₂O₃
Molecular Weight	346.379
Flash Point	222.4±26.4 °C
Exact Mass	346.131744
PSA	87.57000
LogP	1.84
Vapour Pressure	0.0±1.4 mmHg at 25°C
Index of Refraction	1.684
Storage condition	-20°C

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	T+
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3.0
HS Code	2932999099

62669-70-9 structure

62669-70-9

~%

109244-58-8 structure

109244-58-8

Literature: Journal of Medicinal Chemistry, , vol. 30, # 10 p. 1757 - 1761

1250257-82-9 structure

1250257-82-9

~6%

109244-58-8 structure

109244-58-8

Literature: Kundu, Kousik; Knight, Sarah F.; Lee, Seungjun; Taylor, W. Robert; Murthy, Niren Angewandte Chemie - International Edition, 2010 , vol. 49, # 35 p. 6134 - 6138

Precursor 1
62669-70-9
DownStream 5
109282-63-5 109244-59-9 109244-61-3 109244-60-2
109244-62-4

HS Code	2932999099
Summary	2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%