14696-39-0

14696-39-0 structure

Name: Astrophloxine
Chemical Name: 1,1'-diethyl-3,3,3',3'-tetramethylindocarbocyanine iodide
CAS Number: 14696-39-0
Molecular Formula: C₂₇H₃₃IN₂
Molecular Weight: 512.46900
Catalog: Research Areas Others
Create Date: 2018-12-04 18:34:15
Modify Date: 2024-01-11 23:52:01
Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice[1].

Name	1,1'-diethyl-3,3,3',3'-tetramethylindocarbocyanine iodide
Synonyms	1,1'-Diethyl-3,3,3',3'-tetramethylindocarbocyanine Iodide diic2(3) 1,3-Bis-(1-aethyl-3,3-dimethyl-3H-indol-2-yl)-trimethinium,Jodid 1,3-bis-(1-ethyl-3,3-dimethyl-3H-indol-2-yl)-trimethinium,iodide Astrophloxine N,N'-Diethyl-3,3'-tetramethylindocarbocyanine iodide Astrophloxin DILC2(3) 1-Ethyl-2-[3-(1-ethyl-1,3-dihydro-3,3-diMethyl-2H-indol-2-ylidene)-1-propen-1-yl]-3,3-diMethyl-3H-indoliuMIodide SNC 2

Description	Astrophloxine is a fluorescent imaging probe capable of targeting antiparallel dimers. Astrophloxine can be used to detect aggregated Aβ in brain tissue and cerebrospinal fluid samples of Alzheimer's disease (AD) mice[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
Target	Antiparallel dimer[1]
In Vitro	Astrophloxine binds Aβ dimers with stronger fluorescence intensity (A.U.) than binding Aβ monomers[1]. Protocol for CSF analyses[1]: 1.Prepare 1 μL of CSF samples with 1 μL of 10X protease inhibitors and diluted the mixture with 8 μL of binding buffer. Binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride, 10 mM EDTA; pH 7.2). 2.Dilute Astrophloxine to 0.5 μM with binding buffer. 3.Add individual samples and dye to the wells of a 96-well half-area black microplate with a sample to dye ratio of 1:3. Detect at λex=540 nm/λem=570 nm.
In Vivo	Astrophloxine shows more fluorescence (A.U.) in APP/PS1 mice CSF rather in WT mice, and is able to indicate soluble Aβ oligomers in CSF and insoluble plaques in brain tissue[1].
References	[1]. Lee JC, et al. Discovery of Chemicals to Either Clear or Indicate Amyloid Aggregates by Targeting Memory-Impairing Anti-Parallel Aβ Dimers. Angew Chem Int Ed Engl. 2020 Jul 6;59(28):11491-11500.

Hazard Codes	Xi

14134-81-7

122-51-0

~91%

14696-39-0

Literature: Peng, Zheng-Hong Journal of Materials Chemistry, 1996 , vol. 6, # 4 p. 559 - 565