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  • DC Chemicals Limited
  • China
  • Product Name: Daf-2-da
  • Price: ¥Inquiry/100mg ¥Inquiry/250mg ¥Inquiry/1g
  • Purity: 98.0%
  • Stocking Period: 10 Day
  • Contact: Tony Cao

205391-02-2

205391-02-2 structure
205391-02-2 structure
  • Name: 4,5-Diaminofluorescein diacetate
  • Chemical Name: 4,5-Diaminofluorescein diacetate
  • CAS Number: 205391-02-2
  • Molecular Formula: C24H18N2O7
  • Molecular Weight: 446.409
  • Catalog: Dye Reagents
  • Create Date: 2016-09-29 22:40:09
  • Modify Date: 2024-01-05 14:32:50
  • DAF 2DA is most widely probe for NO measurement.

Name 4,5-Diaminofluorescein diacetate
Synonyms 5,6-Diaminofluorescein diacetate
Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-5,6-diamino-
(6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate
5,6-Diamino-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate
DAF 2DA
Description DAF 2DA is most widely probe for NO measurement.
Related Catalog
In Vitro Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).
References

[1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.

Density 1.5±0.1 g/cm3
Boiling Point 719.0±60.0 °C at 760 mmHg
Molecular Formula C24H18N2O7
Molecular Weight 446.409
Flash Point 320.0±29.2 °C
Exact Mass 446.111389
PSA 140.17000
LogP 1.20
Vapour Pressure 0.0±2.3 mmHg at 25°C
Index of Refraction 1.721
Storage condition 2-8℃
Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Risk Phrases R36/38
Safety Phrases 26
RIDADR NONH for all modes of transport