26647-82-5
26647-82-5 structure
- Name: Succinyl phosphonate
- Chemical Name: 4-oxo-4-phosphonobutanoic acid
- CAS Number: 26647-82-5
- Molecular Formula: C4H7O6P
- Molecular Weight: 182.06900
- Catalog: Research Areas Others
- Create Date: 2016-06-06 20:56:04
- Modify Date: 2024-01-13 19:37:10
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Succinyl phosphonate is an inhibitor of the α-ketoglutarate dehydrogenase.
| Name | 4-oxo-4-phosphonobutanoic acid |
|---|---|
| Synonyms |
Butanoic acid,4-oxo-4-phosphono
succinyl phosphonate |
| Description | Succinyl phosphonate is an inhibitor of the α-ketoglutarate dehydrogenase. |
|---|---|
| Related Catalog | |
| In Vitro | Succinyl phosphonate is an α-Ketoglutarate phosphoanalogue. It is found to be an effective inhibitor of α-ketoglutarate oxidative decarboxylation, catalyzed by both muscle and bacterial α-ketoglutarate dehydrogenase complexes, as well as muscle α-ketoglutarate dehydrogenase[1]. At a concentration of 0.01 mM, succinyl phosphonate completely inhibits isolated brain KGDHC even in the presence of a 200-fold higher concentration of its substrate. In cultured human fibroblasts, 0.01 mM succinyl phosphonate produced 70% inhibition of α-ketoglutarate dehydrogenase complex. DESP and TESP are also inhibitory in the cell system, but only after preincubation, suggesting the release of their charged groups by cellular esterases[2]. Succinyl phosphonate inhibits 2-Oxoglutarate dehydrogenase , the first rate-limiting component of the mitochondrial multi-enzyme complex of oxidative decarboxylation of 2-oxoglutarate, in a highly selective and efficient manner[3]. |
| Cell Assay | MTT assay is employed to test cellular viability. Serial dilutions of succinyl phosphonate (0.01-20 mM) are added to cells in fresh culture media. During the medium exchange before the succinyl phosphonate addition, glioblastoma cells are brought to DMEM with 1 g/L glucose, 1 mM pyruvate and 2 mM glutamax. The influence of succinyl phosphonate in minimal medium is studied in the earlier employed buffered salt solution[3]. |
| References |
| Molecular Formula | C4H7O6P |
|---|---|
| Molecular Weight | 182.06900 |
| Exact Mass | 181.99800 |
| PSA | 121.71000 |
| Storage condition | 2-8℃ |