202850-01-9

202850-01-9 structure

Name: N-[12-[(7-NITRO-2-1,3-BENZOXADIAZOL-4-YL)AMINO]DODECANOYL]-CERAMIDE
Chemical Name: N-[1-hydroxy-14-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]tetradec-4-en-2-yl]hexadecanamide
CAS Number: 202850-01-9
Molecular Formula: C₃₆H₆₁N₅O₆
Molecular Weight: 659.899
Catalog: Research Areas Others
Create Date: 2016-10-20 12:23:21
Modify Date: 2024-02-03 10:32:11
C12-NBD-ceramide is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays[1][2].

Name	N-[1-hydroxy-14-[(4-nitro-2,1,3-benzoxadiazol-7-yl)amino]tetradec-4-en-2-yl]hexadecanamide
Synonyms	Dodecanamide, N-[(1S,2R,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- N-[(2S,3R,4E)-1,3-Dihydroxy-4-octadecen-2-yl]-12-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanamide

Description	C12-NBD-ceramide is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). In this experiment, C12-NBD-ceramide is used as a fluorescent nCDase substrate and reaction products will be separated by reverse-phase HPLC[2]: 1. 20 µM substrate, 1 nM nCDase in 75 mM NaCl, 25 mM HEPES (pH 8.0), and 0.4% Triton X-100, for 2 h at 37°C in a final volume of 100 µL. 2. Extract samples with 1:1 chloroform-methanol dries under nitrogen gas, and resuspends in 60 µL of HPLC mobile phase B. Separats reaction products by reverse-phase HPLC using a Spectra 3 µm C8SR column (3 μm particle, 3.0 × 150 mm). 3. Mobile phase A contains 0.2% formic acid and 1 mM ammonium formate in HPLC-grade water. Mobile phase B contains 0.2% formic acid (IL) and 1 mM ammonium formate in HPLC-grade methanol. 4. FRET-based assay using 20 µM substrate ES.173.cds, 50 ng of nCDase, 75 mM NaCl, 15 mM sodium phosphate (pH 7.4), and 0.3% Triton X-100 for 3 h in 96 black well plates. 5. Measure resorufin fluorescence (Ex=347 nm, Em=430 and 530 nm).
References	[1]. Tani M, et al. Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. J Biochem. 1999 Apr;125(4):746-9. [2]. Otsuka Y, et al. Identification of Small-Molecule Inhibitors of Neutral Ceramidase (nCDase) via Target-Based High-Throughput Screening. SLAS Discov. 2021 Jan;26(1):113-121.

Density	1.1±0.1 g/cm3
Molecular Formula	C₃₆H₆₁N₅O₆
Molecular Weight	659.899
Exact Mass	659.462158
PSA	166.33000
LogP	11.44
Index of Refraction	1.547

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