Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Ingenol 3-mebutate
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Purity: 98.0%
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Shanghai Jizhi Biochemical Technology Co., Ltd
China
Product Name: Ingenol 3-mebutate
Price: ￥3928.0/20mg
Purity: 98.0%
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DC Chemicals Limited
China
Product Name: Ingenol 3-angelate
Price: $800.0/20mg $1600.0/50mg
Purity: 98.0%
Stocking Period: 3 Day
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Dayang Chem (Hangzhou) Co., Ltd.
China
Product Name: Ingenol-3-angelate
Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
Purity: 98.0%
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75567-37-2

75567-37-2 structure

Name: Ingenol 3-mebutate
Chemical Name: ingenol mebutate
CAS Number: 75567-37-2
Molecular Formula: C₂₅H₃₄O₆
Molecular Weight: 430.534
Catalog: Biochemical Natural product
Create Date: 2018-08-14 23:00:13
Modify Date: 2024-01-03 09:49:11
Ingenol Mebutate is an active ingredient in Euphorbia peplus, acts as a potent PKC modulator, with Kis of 0.3, 0.105, 0.162, 0.376, and 0.171 nM for PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε, respectively, and has antiinflammatory and antitumor activity.

Name	ingenol mebutate
Synonyms	Indoxyl \|A-D-galactopyranoside 3-O-angeloylingenol 2-Butenoic acid, 2-methyl-, (1aR,2R,5R,5aS,6S,8aS,9R,10aR)-1a,2,5,5a,6,9,10,10a-octahydro-5,5a-dihydroxy-4-(hydroxymethyl)-1,1,7,9-tetramethyl-11-oxo-1H-2,8a-methanocyclopenta[a]cyclopropa[e]cyclodecen-6-yl ester, (2Z)- Ingenol 3-angelate 3-Indolyl-b-D-galactopyranoside (1S,4S,5S,6R,9R,10R,12R,14R)-5,6-Dihydroxy-7-(hydroxymethyl)-3,11,11,14-tetramethyl-15-oxotetracyclo[7.5.1.0<sup>1,5</sup>.0<sup>10,12</sup>]pentadeca-2,7-dien-4-yl (2Z)-2-methyl-2-butenoate indican (glucoside) Picato Ingenol mebutate (1S,4S,5S,6R,9R,10R,12R,14R)-5,6-Dihydroxy-7-(hydroxymethyl)-3,11,11,14-tetramethyl-15-oxotetracyclo[7.5.1.0.0]pentadeca-2,7-dien-4-yl (2Z)-2-methyl-2-butenoate Ingenol-3-angelate 3-Ingenyl angelate 3-Angeloylingenol

Description	Ingenol Mebutate is an active ingredient in Euphorbia peplus, acts as a potent PKC modulator, with Kis of 0.3, 0.105, 0.162, 0.376, and 0.171 nM for PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε, respectively, and has antiinflammatory and antitumor activity.
Related Catalog	Signaling Pathways >> Epigenetics >> PKC Signaling Pathways >> TGF-beta/Smad >> PKC Natural Products >> Terpenoids and Glycosides Research Areas >> Cancer Research Areas >> Inflammation/Immunology
Target	PKC-β:0.105 nM (Ki) PKC-γ:0.162 nM (Ki) PKC-ε:0.171 nM (Ki) PKC-α:0.3 nM (Ki) PKC-δ:0.376 nM (Ki)
In Vitro	Ingenol Mebutate (Ingenol 3-angelate) is an active ingredient in Euphorbia peplus, acting as a potent PKC activator, with Kis of 0.3, 0.105, 0.162, 0.376, and 0.171 nM for PKC-α, PKC-β, PKC-γ, PKC-δ, and PKC-ε, respectively. Ingenol Mebutate also EC50s of 13 ± 2.4 nM (PKC-α), 4.37 ± 0.4 nM (PKC-βI), 10.5 ± 2.2 nM (PKC-βII), 38.6 ± 2.9 nM (PKC-δ), 1.08 ± 0.01 nM (PKC-ε), 0.9 ± 0.13 nM (PKC-μ) in WEHI-231 cells, 198 ± 12.5 nM (PKC-α), 69.1 ± 8.2 nM (PKC-βI), 4.6 ± 0.4 nM (PKC-ε) and 1 nM (PKC-μ) in HOP-92 cells, 635 ± 245 nM (PKC-α), 146 ± 35 nM (PKC-βI), 4.7 ± 0.7 nM (PKC-δ), 1.1 ± 0.5 nM (PKC-ε), and 30 nM (PKC-μ) in Colo-205 cells. Ingenol Mebutate sensitizes WEHI-231 cells, HOP-92 and Colo-205 cells, with IC50s of 1.41 ± 0.255 nM, 3.24 ± 2.01 nM, and 11.9 ± 1.307 nM, respectively[1]. Ingenol Mebutate (PEP005; 20 nM) actions are PKC-δ dependent, induces apoptosis in primary AML marrow blasts but not in normal myeloblasts[2]. Ingenol Mebutate (PEP005) activates PKCδ and inhibits PKCα. Colo205-R cells (IC50: >10 μM) are >300-fold more resistant to Ingenol Mebutate than parental Colo205-S cells[3].
Cell Assay	KG1a cells are transiently transfected with EGFP-tagged mouse PKC-δ subcloned into pEGFP-N1 plasmid using an Amaxa nucleofection apparatus. Cells are treated with Ingenol Mebutate (0.2 μM-20 μM) 24 hours after transfection. Cell viability in EGFP-positive cells is assessed and loss of viability confirmed in the total cell culture by MTT assay after 3 days. Briefly, 24 hours after transfection, 2 × 104 cells are plated in 5 wells in 96-well plates and exposed to 0, 0.2, 2, and 20 μM Ingenol Mebutate. At 72 hours, 20 μL MTT substrate at 5 mg/mL is added and plates are incubated at 37°C. After 3 hours, 150 μL media is removed and replaced with 200 μL dimethyl sulfoxide (DMSO). Absorbance at an optical density (OD) of 550 nm is read on a plate reader and corrected for absorbance obtained from blank media controls[2].
References	[1]. Kedei N, et al. Characterization of the interaction of ingenol 3-angelate with protein kinase C. Cancer Res. 2004 May 1;64(9):3243-55. [2]. Hampson P, et al. PEP005, a selective small-molecule activator of protein kinase C, has potent antileukemic activity mediated via the delta isoform of PKC. Blood. 2005 Aug 15;106(4):1362-8. [3]. Ghoul A, et al. Epithelial-to-mesenchymal transition and resistance to ingenol 3-angelate, a novel protein kinase C modulator, in colon cancer cells. Cancer Res. 2009 May 15;69(10):4260-9.

Density	1.3±0.1 g/cm3
Boiling Point	576.9±50.0 °C at 760 mmHg
Molecular Formula	C₂₅H₃₄O₆
Molecular Weight	430.534
Flash Point	191.4±23.6 °C
Exact Mass	430.235535
PSA	104.06000
LogP	5.18
Appearance	white to beige
Vapour Pressure	0.0±3.6 mmHg at 25°C
Index of Refraction	1.594
Storage condition	?20°C
Water Solubility	DMSO: soluble15mg/mL, clear \| Soluble in 100% ethanol, DMSO, dichloromethane, and methanol. Insoluble in water.

RIDADR	NONH for all modes of transport

Precursor 7
1356187-24-0 1356187-19-3 565-63-9 77573-43-4
30220-46-3 94487-74-8 1356187-16-0
DownStream 0

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