Indo-1 AM
Indo-1 AM structure
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Common Name | Indo-1 AM | ||
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| CAS Number | 112926-02-0 | Molecular Weight | 1009.91000 | |
| Density | 1.374g/cm3 | Boiling Point | 1009.2ºC at 760mmHg | |
| Molecular Formula | C47H51N3O22 | Melting Point | N/A | |
| MSDS | USA | Flash Point | 564.1ºC | |
Use of Indo-1 AMIndo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm). |
| Name | acetyloxymethyl 2-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-indole-6-carboxylate |
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| Synonym | More Synonyms |
| Description | Indo-1 AM is a fluorescent Ca2+ indicator (λex=340 nm, λem=405/485 nm). |
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| Related Catalog | |
| In Vitro | Indo-1 AM is a fluorescent Ca2+ indicator with λex=340 nm and λem=405/485 nm[1]. In rabbit hearts that are exposed to Indo-1 AM, 72% of the dye is in the cytosol, and the remainder is associated with the mitochondria, microsomes and the nucleus[2]. |
| Cell Assay | The cells are loaded with indo-I and fura-2 by exposure to the membrane-permeant forms, Indo-1 AM (indo-l-acetoxymethyl ester) and fura-2-acetoxymethyl ester, respectively. A mixture of 10 μL of 1 mM Indo-1 AM or fura-2/AM in dimethylsulfoxide (DMSO), 2.5 μL of 25% wt/wt Pluronic F-127 in DMSO, and 75 μL fetal calf serum (FCS) or newborn calf serum (NCS) is added to 2 mL Tyrode solution containing the cells and 40 μL FCS or NCS. Loading is achieved by gently shaking the cells for 15 to 30 min[2]. |
| References |
| Density | 1.374g/cm3 |
|---|---|
| Boiling Point | 1009.2ºC at 760mmHg |
| Molecular Formula | C47H51N3O22 |
| Molecular Weight | 1009.91000 |
| Flash Point | 564.1ºC |
| Exact Mass | 1009.30000 |
| PSA | 303.73000 |
| LogP | 3.14650 |
| Vapour Pressure | 3.2E-28mmHg at 25°C |
| Index of Refraction | 1.577 |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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| Safety Phrases | S22-S24/25 |
| RIDADR | NONH for all modes of transport |
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Quantitative TCR:pMHC Dissociation Rate Assessment by NTAmers Reveals Antimelanoma T Cell Repertoires Enriched for High Functional Competence.
J. Immunol. 195 , 356-66, (2015) Experimental models demonstrated that therapeutic induction of CD8 T cell responses may offer protection against tumors or infectious diseases providing that T cells have sufficiently high TCR/CD8:pMH... |
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Identification of Rare High-Avidity, Tumor-Reactive CD8+ T Cells by Monomeric TCR-Ligand Off-Rates Measurements on Living Cells.
Cancer Res. 75 , 1983-91, (2015) The avidity of the T-cell receptor (TCR) for antigenic peptides presented by the peptide-MHC (pMHC) on cells is a key parameter for cell-mediated immunity. Yet a fundamental feature of most tumor anti... |
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Pharmacological analysis by HOE642 and KB-R9032 of the role of Na(+)/H(+) exchange in the endothelin-1-induced Ca(2+) signalling in rabbit ventricular myocytes.
Br. J. Pharmacol. 131(3) , 638-44, (2000) The role of Na(+)/H(+) exchange in endothelin-1 (ET-1)-induced increases in Ca(2+) transients and cell shortening was studied in rabbit ventricular myocytes loaded with indo-1/AM. Selective inhibitors... |
| MFCD00036955 |
| Indo-1 pentaacetoxymethyl ester |
| INDO 1/AM |
| Indo-1 AM |