![]() Mag-indo-1/AM structure
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Common Name | Mag-indo-1/AM | ||
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CAS Number | 130926-94-2 | Molecular Weight | 612.58100 | |
Density | 1.335g/cm3 | Boiling Point | 789.9ºC at 760mmHg | |
Molecular Formula | C30H32N2O12 | Melting Point | N/A | |
MSDS | Chinese USA | Flash Point | 431.5ºC |
Use of Mag-indo-1/AMMag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2]. |
Name | acetyloxymethyl 2-[4-[bis[2-oxo-2-(2-oxopropoxymethoxy)ethyl]amino]phenyl]-1H-indole-6-carboxylate |
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Synonym | More Synonyms |
Description | Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2]. |
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Related Catalog | |
In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Measurement of intracellular Ca2+[1]: 1. Incubate 3 mL cell suspension with 10 µM Mag-indo-1/AM for 15 min at 30℃. 2. Centrifuge the cells for 1 min at 500 rpm and resuspend in 5 mL isolation buffer (containing 1 mM CaCl2). 3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol and store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use. 4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend and store at room temperature. 6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope. 7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm (the peak emissions of the Ca2+ bound and Ca2+ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system. |
References |
Density | 1.335g/cm3 |
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Boiling Point | 789.9ºC at 760mmHg |
Molecular Formula | C30H32N2O12 |
Molecular Weight | 612.58100 |
Flash Point | 431.5ºC |
Exact Mass | 612.19600 |
PSA | 176.83000 |
LogP | 2.53130 |
Vapour Pressure | 6.32E-25mmHg at 25°C |
Index of Refraction | 1.585 |
Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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RIDADR | NONH for all modes of transport |
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