L-Azidonorleucine hydrochloride structure
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Common Name | L-Azidonorleucine hydrochloride | ||
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CAS Number | 1454334-76-9 | Molecular Weight | 208.646 | |
Density | N/A | Boiling Point | N/A | |
Molecular Formula | C6H13ClN4O2 | Melting Point | N/A | |
MSDS | N/A | Flash Point | N/A |
Use of L-Azidonorleucine hydrochlorideL-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2]. |
Name | (1S)-5-Azido-1-carboxy-1-pentanaminium chloride |
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Synonym | More Synonyms |
Description | L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2]. |
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Related Catalog | |
In Vitro | CHO cells transfected with pMaRSC and incubated with L-Azidonorleucine (Anl; 1.5 mM; 6 h) are fixed, permeabilized, and treated with alkyne-TAMRA. The TAMRA fluorescence exclusively in the mCherry-expressing cells is observed, consistent with cell-selective incorporation of Anl into cellular proteins[1]. |
In Vivo | When L-Azidonorleucine (ANL) is administered via the drinking water, an average daily L-Azidonorleucine intake of 0.9 mg/day/g of body weight for 21 d when aiming to label and identify neuronal populations of the brain are recommended. Lower dosages or shorter labeling times might be used for the labeling of other cell types. When L-Azidonorleucine is administered by i.p. injection, the testing different L-Azidonorleucine doses in the range of 4-400 mM are recommended. A 400 mM dosage administered by i.p. (i.p. volume: 10 ml/kg) once per day for 1 week is recommended for labeling some neuronal populations (such as excitatory neurons of the hippocampus). Lower dosages (e.g., 4 mM) can potentially be used for labeling other cell types or when longer labeling periods are desired[2]. |
References |
Molecular Formula | C6H13ClN4O2 |
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Molecular Weight | 208.646 |
Exact Mass | 208.072708 |
Hazard Codes | Xi |
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MFCD18382050 |
MFCD18382040 |
(1S)-5-Azido-1-carboxy-1-pentanaminium chloride |
L-Norleucine, 6-azido-, hydrochloride (1:1) |