1454334-76-9

1454334-76-9 structure

Name: L-Azidonorleucine hydrochloride
Chemical Name: (1S)-5-Azido-1-carboxy-1-pentanaminium chloride
CAS Number: 1454334-76-9
Molecular Formula: C₆H₁₃ClN₄O₂
Molecular Weight: 208.646
Catalog: Research Areas Others
Create Date: 2020-04-05 15:16:03
Modify Date: 2024-01-12 20:59:35
L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2].

Name	(1S)-5-Azido-1-carboxy-1-pentanaminium chloride
Synonyms	MFCD18382050 MFCD18382040 (1S)-5-Azido-1-carboxy-1-pentanaminium chloride L-Norleucine, 6-azido-, hydrochloride (1:1)

Description	L-Azidonorleucine hydrochloride, an unnatural amino acid, is A Methionine surrogate. L-Azidonorleucine hydrochloride can be used to label mammalian cell proteins and identify a diverse set of methionyl-tRNA synthetase (MetRS) mutants [1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	CHO cells transfected with pMaRSC and incubated with L-Azidonorleucine (Anl; 1.5 mM; 6 h) are fixed, permeabilized, and treated with alkyne-TAMRA. The TAMRA fluorescence exclusively in the mCherry-expressing cells is observed, consistent with cell-selective incorporation of Anl into cellular proteins[1].
In Vivo	When L-Azidonorleucine (ANL) is administered via the drinking water, an average daily L-Azidonorleucine intake of 0.9 mg/day/g of body weight for 21 d when aiming to label and identify neuronal populations of the brain are recommended. Lower dosages or shorter labeling times might be used for the labeling of other cell types. When L-Azidonorleucine is administered by i.p. injection, the testing different L-Azidonorleucine doses in the range of 4-400 mM are recommended. A 400 mM dosage administered by i.p. (i.p. volume: 10 ml/kg) once per day for 1 week is recommended for labeling some neuronal populations (such as excitatory neurons of the hippocampus). Lower dosages (e.g., 4 mM) can potentially be used for labeling other cell types or when longer labeling periods are desired[2].
References	[1]. Alborz Mahdavi, et al. Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of Mammalian Protein Synthesis. J Am Chem Soc. 2016 Apr 6;138(13):4278-81. [2]. Beatriz Alvarez-Castelao, et al. Cell-type-specific metabolic labeling, detection and identification of nascent proteomes in vivo. Nat Protoc. 2019 Feb;14(2):556-575.

Molecular Formula	C₆H₁₃ClN₄O₂
Molecular Weight	208.646
Exact Mass	208.072708

Hazard Codes	Xi

1454334-76-9	135340-91-9
130397-01-2	71795-47-6
821788-01-6	2173-47-9
2014-27-9	2409-35-0
2014-30-4	6003-05-0
2746377-00-2	1158522-08-7
2770580-98-6	940364-43-2
134104-43-1	2742060-30-4
2770640-74-7	131615-57-1
2748578-88-1	6906-52-1