NAI-N3

Modify Date: 2024-01-08 16:32:29

NAI-N3 Structure
NAI-N3 structure
Common Name NAI-N3
CAS Number 1612756-29-2 Molecular Weight 228.21
Density N/A Boiling Point N/A
Molecular Formula C10H8N6O Melting Point N/A
MSDS N/A Flash Point N/A

 Use of NAI-N3


NAI-N3 is a novel RNA acylation reagent that enables RNA purification.

 Names

Name NAI-N3

 NAI-N3 Biological Activity

Description NAI-N3 is a novel RNA acylation reagent that enables RNA purification.
Related Catalog
In Vitro The bifunctional NAI-N3 SHAPE reagent is used to label all RNAs inside living cells (or in vitro refolded RNA) to generate covalent modifications on targeted RNAs at single-nucleotide resolution. RNA is isolated, biotinylated using click chemistry and randomly fragmented to a size of 50–100 nt. To generate cDNA sequencing libraries, the RNA fragments are subjected to 3′-end repair, adapter ligation, size selection, biotin enrichment, reverse transcription (RT) and PCR. The end result is selective amplification of fragments corresponding to modified bases in the NAI-N3–treated samples or background expression values from mock-treated samples. Sequencing reads generated from the NAI-N3– and mock (DMSO)-treated samples are mapped to a transcriptome annotation, followed by computational identification of RT stops corresponding to SHAPE modifications—and hence reactive nucleotides—across the entire transcriptome[1].
References

[1]. Flynn RA, et al. Transcriptome-wide interrogation of RNA secondary structure in living cells with icSHAPE. Nat Protoc. 2016 Feb;11(2):273-90.

 Chemical & Physical Properties

Molecular Formula C10H8N6O
Molecular Weight 228.21
Storage condition 2-8℃
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Price: $92/10mM*1mLinDMSO

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