Fura-FF pentapotassium

Modify Date: 2024-01-12 12:44:10

Fura-FF pentapotassium structure	Common Name	Fura-FF pentapotassium
	CAS Number	192140-58-2	Molecular Weight	853.9425264
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₂₈H₁₈F₂K₅N₃O₁₄	Melting Point	>250℃
	MSDS	N/A	Flash Point	N/A

Use of Fura-FF pentapotassium

Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration)[1].

Name	Fura-FF (potassium salt)

Description	Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration)[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Human Sperm Cells: 1. Incubate the cells according to your normal protocol. 2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 ℃ for 40 min. 3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 1 ◊ 107 cells in 3 mL of H-HSM and maintained at 36 ℃. 4. Immediately measure fluorescence recordings.
References	[1]. Ruggiu AA, et al. Fura-2FF-based calcium indicator for protein labeling. Org Biomol Chem. 2010 Aug 7;8(15):3398-401. [2]. Neri-Vidaurri Pdel C, et al. A remarkable increase in the pHi sensitivity of voltage-dependent calcium channels occurs in human sperm incubated in capacitating conditions. Biochem Biophys Res Commun. 2006 Apr 28;343(1):105-9.

Melting Point	>250℃
Molecular Formula	C₂₈H₁₈F₂K₅N₃O₁₄
Molecular Weight	853.9425264
Storage condition	−20°C
Water Solubility	H2O: soluble

Safety Phrases	22-24/25
WGK Germany	3