192140-58-2

192140-58-2 structure
192140-58-2 structure
  • Name: Fura-FF pentapotassium
  • Chemical Name: Fura-FF (potassium salt)
  • CAS Number: 192140-58-2
  • Molecular Formula: C28H18F2K5N3O14
  • Molecular Weight: 853.9425264
  • Catalog: Research Areas Others
  • Create Date: 2021-04-29 18:57:00
  • Modify Date: 2024-01-12 12:44:10
  • Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration)[1].

Name Fura-FF (potassium salt)
Description Fura-FF pentapotassium is a low-affinity fluorescent dye for calcium (Ex/Em: 365/514 nm in the absence of calcium; 339/507 nm in the presence of a high calcium concentration)[1].
Related Catalog
In Vitro Guidelines[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Human Sperm Cells: 1. Incubate the cells according to your normal protocol. 2. Load cells with 5 μM Fura-FF in 1 mL Hepes-buffered human sperm (H-HSM) medium at 36 ℃ for 40 min. 3. Wash the sample by centrifugation and scatter cells in disposable centrifuge tubes at 1 ◊ 107 cells in 3 mL of H-HSM and maintained at 36 ℃. 4. Immediately measure fluorescence recordings.
References

[1]. Ruggiu AA, et al. Fura-2FF-based calcium indicator for protein labeling. Org Biomol Chem. 2010 Aug 7;8(15):3398-401.

[2]. Neri-Vidaurri Pdel C, et al. A remarkable increase in the pHi sensitivity of voltage-dependent calcium channels occurs in human sperm incubated in capacitating conditions. Biochem Biophys Res Commun. 2006 Apr 28;343(1):105-9.

Melting Point >250℃
Molecular Formula C28H18F2K5N3O14
Molecular Weight 853.9425264
Storage condition −20°C
Water Solubility H2O: soluble
Safety Phrases 22-24/25
WGK Germany 3