IR-813 perchlorate

Modify Date: 2024-01-03 01:44:34

IR-813 perchlorate Structure
IR-813 perchlorate structure
Common Name IR-813 perchlorate
CAS Number 201024-57-9 Molecular Weight 633.60400
Density N/A Boiling Point N/A
Molecular Formula C36H38Cl2N2O4 Melting Point 238-242ºC
MSDS Chinese USA Flash Point N/A

 Use of IR-813 perchlorate


IR 813 perchlorate is a near-infrared (NIR) fluorescent dye (Ex=815 nm, Em=840 nm) and can be used for visualizing regional lymph nodes in mice[1].

 Names

Name ir-813 perchlorate
Synonym More Synonyms

 IR-813 perchlorate Biological Activity

Description IR 813 perchlorate is a near-infrared (NIR) fluorescent dye (Ex=815 nm, Em=840 nm) and can be used for visualizing regional lymph nodes in mice[1].
Related Catalog
In Vitro IR 813 (17.3 μM, 2 h) induces a 31.4% hemolysis in red blood cells[1]. IR 813 (5.9 μM, 24 h) induces 50% cell death in MRC-5 cells[1].
In Vivo IR 813 perchlorate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice[1]. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum. 2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice. 3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days). 4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.
References

[1]. Marion Hell, et al. Surface chemistry architecture of silica nanoparticles determine the efficiency of in vivo fluorescence lymph node mapping. ACS Nano. 2013 Oct 22;7(10):8645-57.

 Chemical & Physical Properties

Melting Point 238-242ºC
Molecular Formula C36H38Cl2N2O4
Molecular Weight 633.60400
Exact Mass 632.22100
PSA 80.52000
LogP 9.55480

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Safety Phrases S22-S24/25

 Synonyms

IR-813 perchlorate,2-[2-[2-Chloro-3-[2-(1,3-dihydro-1,1,3-trimethyl-2H-benzo[e]-indol-2-ylidene)-ethylidene]-1-cyclohexen-1-yl]-ethenyl]-1,1,3-trimethyl-1H-benzo[e]indolium perchlorate
2-[2-[2-chloro-3-[2-(1,3-dihydro-1,1,3-trimethyl-2h-benzo[e]-indol-2-ylidene)-ethylidene]-1-cyclohexen-1-yl]-ethenyl]-1,1,3-trimethyl-1h-benzo[e]indolium perchlorate