Hoechst 33342

Modify Date: 2024-01-06 00:26:42

Hoechst 33342 structure	Common Name	Hoechst 33342
	CAS Number	23491-52-3	Molecular Weight	452.55
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₂₇H₂₈N₆O	Melting Point	268ºC
	MSDS	Chinese USA	Flash Point	N/A
	Symbol	GHS07, GHS08	Signal Word	Warning

Use of Hoechst 33342

Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

Name	2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole
Synonym	More Synonyms

Description	Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
Related Catalog	Signaling Pathways >> Autophagy >> Autophagy Dye Reagents Research Areas >> Others
Target	Dye reagent[1] DNA Stain[1]
In Vitro	Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].
Cell Assay	Labeling Nuclear DNA with Hoechst 33342[1]Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+.Step 5, Mount the coverslips.Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].
References	[1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

Melting Point	268ºC
Molecular Formula	C₂₇H₂₈N₆O
Molecular Weight	452.55
PSA	100.76000
LogP	7.13970
Storage condition	2-8°C

Hoechst 33342 MSDS(Chinese)

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DT4200000

CHEMICAL NAME :: 2,5'-Bibenzimidazole, 2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-

CAS REGISTRY NUMBER :: 23491-52-3

LAST UPDATED :: 199312

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: DNA inhibition

TEST SYSTEM :: Rodent - hamster Lung

DOSE/DURATION :: 1 umol/L

REFERENCE :: JHCYAS Journal of Histochemistry and Cytochemistry. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1953- Volume(issue)/page/year: 30,111,1982

Symbol	GHS07, GHS08
Signal Word	Warning
Hazard Statements	H302-H315-H335-H341
Precautionary Statements	P280-P301 + P312 + P330
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xn: Harmful;
Risk Phrases	R22
Safety Phrases	S22-S36/37-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	SM1140500
HS Code	2933990090

4-Ethoxybenzaldehyde structure

4-Ethoxybenzaldehyde

CAS#:10031-82-0

1,2-Benzenediamine, 4-[6-(4-Methyl-1-piperazinyl)-1H-benzimidazol-2-yl]- structure

1,2-Benzenediam...

CAS#:23491-49-8

~82%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Harapanhalli; McLaughlin; Howell; Rao; Adelstein; Kassis Journal of medicinal chemistry, 1996 , vol. 39, # 24 p. 4804 - 4809

5-Chloro-2-nitroaniline structure

5-Chloro-2-nitr...

CAS#:1635-61-6

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Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

methylpiperazine structure

methylpiperazine

CAS#:109-01-3

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

4-cyano-2-nitrotrifluoroacetanilide structure

4-cyano-2-nitro...

CAS#:184033-32-7

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

4-[5'-(4''-methylpiperazin-1''-yl)benzimidazol-2'-yl]-2-nitroaniline structure

4-[5'-(4''-meth...

CAS#:23623-05-4

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

4-Amino-3-nitrobenzonitrile structure

4-Amino-3-nitro...

CAS#:6393-40-4

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

4-(4-Methylpiperazino)-1,2-benzenediamine structure

4-(4-Methylpipe...

CAS#:54998-08-2

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

5-(4-Methylpiperazin-1-yl)-2-nitroaniline structure

5-(4-Methylpipe...

CAS#:23491-48-7

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

4-hydroxybenzaldehyde structure

4-hydroxybenzal...

CAS#:123-08-0

~%

Hoechst 33342 Structure

Hoechst 33342

CAS#:23491-52-3

Literature: Journal of medicinal chemistry, , vol. 39, # 24 p. 4804 - 4809

HS Code	2933990090
Summary	2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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bisBenzimide H 33342 trihydrochloride

2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, trihydrochloride

2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole

2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride

2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole

2,6'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-, hydrochloride (1:3)

MFCD00166996

BISBENZIMIDE ETHOXIDE TRIHYDROCHLORIDE

2,5'-bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, hydrochloride (1:3)

Hoe 33342

2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride

EINECS 245-691-1

Hoechst 33342

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