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23491-52-3

23491-52-3 structure
23491-52-3 structure
  • Name: Hoechst 33342
  • Chemical Name: 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole
  • CAS Number: 23491-52-3
  • Molecular Formula: C27H28N6O
  • Molecular Weight: 452.55
  • Catalog: Dye Reagents
  • Create Date: 2018-06-25 17:27:31
  • Modify Date: 2024-01-06 00:26:42
  • Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
Name 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole
Synonyms bisBenzimide H 33342 trihydrochloride
2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, trihydrochloride
2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole
2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride
2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole
2,6'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-, hydrochloride (1:3)
MFCD00166996
BISBENZIMIDE ETHOXIDE TRIHYDROCHLORIDE
2,5'-bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, hydrochloride (1:3)
Hoe 33342
2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride
EINECS 245-691-1
Hoechst 33342
Description Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
Related Catalog
Target

Dye reagent[1] DNA Stain[1]

In Vitro Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].
Cell Assay Labeling Nuclear DNA with Hoechst 33342[1]Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+.Step 5, Mount the coverslips.Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].
References

[1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

Melting Point 268ºC
Molecular Formula C27H28N6O
Molecular Weight 452.55
PSA 100.76000
LogP 7.13970
Storage condition 2-8°C

CHEMICAL IDENTIFICATION

RTECS NUMBER :
DT4200000
CHEMICAL NAME :
2,5'-Bibenzimidazole, 2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-
CAS REGISTRY NUMBER :
23491-52-3
LAST UPDATED :
199312

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :
DNA inhibition
TEST SYSTEM :
Rodent - hamster Lung
DOSE/DURATION :
1 umol/L
REFERENCE :
JHCYAS Journal of Histochemistry and Cytochemistry. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1953- Volume(issue)/page/year: 30,111,1982
Symbol GHS07 GHS08
GHS07, GHS08
Signal Word Warning
Hazard Statements H302-H315-H335-H341
Precautionary Statements P280-P301 + P312 + P330
Personal Protective Equipment dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes Xn: Harmful;
Risk Phrases R22
Safety Phrases S22-S36/37-S24/25
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS SM1140500
HS Code 2933990090
HS Code 2933990090
Summary 2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Chemsrc provides CAS#:23491-52-3 MSDS, density, melting point, boiling point, structure, formula, molecular weight, synthetic route, etc.
title: CAS No. 23491-52-3 | Chemsrc address: https://www.chemsrc.com/en/baike/1182344.html

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