ZMF-10

Modify Date: 2024-01-06 15:22:55

ZMF-10 structure	Common Name	ZMF-10
	CAS Number	2415295-37-1	Molecular Weight	473.37
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₁₉H₁₇F₆N₇O	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of ZMF-10

ZMF-10 is a highly potent PAK1 inhibitor, with IC50s of 174 nM, 1.038 μM and 1.372 μM for PAK1, PAK2 and PAK3, respectively. ZMF-10 can inhibit PAK1 activity to affect PAK1-regulated apoptosis, ER-Stress and migration in MDA-MB-231 cells. ZMF-10 can be used for researching anticancer[1].

Name	ZMF-10

Description	ZMF-10 is a highly potent PAK1 inhibitor, with IC50s of 174 nM, 1.038 μM and 1.372 μM for PAK1, PAK2 and PAK3, respectively. ZMF-10 can inhibit PAK1 activity to affect PAK1-regulated apoptosis, ER-Stress and migration in MDA-MB-231 cells. ZMF-10 can be used for researching anticancer[1].
Related Catalog	Signaling Pathways >> Apoptosis >> Apoptosis Research Areas >> Cancer Signaling Pathways >> Cytoskeleton >> PAK Signaling Pathways >> Cell Cycle/DNA Damage >> PAK
Target	PAK1:174 nM (IC50) PAK2:1.038 μM (IC50) PAK3:1.372 μM (IC50)
In Vitro	ZMF-10 (0-10 μM; 48 hours) exhibits potent antiproliferative activity in a dose-dependent manner[1]. ZMF-10 (20 μM; 24 hours) suppresses the phosphorylation of PAK1 at Ser199 and Thr212[1]. ZMF-10 (10-40 μM; 24 hours) downregulates the expression of ERK, and suppresses the phosphorylation of c-Raf, MEK and ERK; up-regulates the expression of Bax and downregulates the expression of Bcl-2; induces apoptosis in a dose-dependent manner with the increase of early and late apoptotic cell population[1]. ZMF-10 (10-40 μM; 24 hours) inhibits the PI3K-AKT-mTOR signaling with the decreasing phosphorylation of AKT and mTOR; inhibits JNK1/2 and cells migration in MDA-MB-231 cells[1]. Cell Proliferation Assay Cell Line: MDA-MB-231[1] Concentration: 0-10 μM Incubation Time: 48 hours Result: Exhibited potent antiproliferative activity in a dose-dependent manner with an IC50 value of 3.48 μM. Immunofluorescence Cell Line: MDA-MB-231[1] Concentration: 20 μM Incubation Time: 24 hours Result: Suppressed the phosphorylation of PAK1 at Ser199 and Thr212. Western Blot Analysis Cell Line: MDA-MB-231[1] Concentration: 10, 20 and 40 μM Incubation Time: 24 hours Result: Downregulated the expression of ERK, and suppressed the phosphorylation of c-Raf, MEK and ERK; up-regulated the expression of Bax and downregulated the expression of Bcl-2. Apoptosis Analysis Cell Line: MDA-MB-231[1] Concentration: 10, 20 and 40 μM Incubation Time: 24 hours Result: Induced apoptosis in a dose-dependent manner with the increase of early and late apoptotic cell population.
References	[1]. Zhang J, et al. Design, synthesis and biological evaluation of 1H-pyrazolo [3,4-d]pyrimidine derivatives as PAK1 inhibitors that trigger apoptosis, ER stress and anti-migration effect in MDA-MB-231 cells. Eur J Med Chem. 2020;194:112220.

Molecular Formula	C₁₉H₁₇F₆N₇O
Molecular Weight	473.37

ZMF-10

Use of ZMF-10

Names

ZMF-10 Biological Activity

Chemical & Physical Properties