NHC-triphosphate
Modify Date: 2024-01-07 16:09:29
NHC-triphosphate structure
|
Common Name | NHC-triphosphate | ||
|---|---|---|---|---|
| CAS Number | 34973-27-8 | Molecular Weight | 499.15600 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C9H16N3O15P3 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of NHC-triphosphateNHC-triphosphate is an intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) as a triphosphate form. NHC-triphosphate is a weak alternative substrate for the viral polymerase and changes the mobility of the product in polyacrylamide electrophoresis gels[1]. |
| Name | [[(2R,3S,4R,5R)-3,4-dihydroxy-5-[4-(hydroxyamino)-2-oxopyrimidin-1-yl]oxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate |
|---|---|
| Synonym | More Synonyms |
| Description | NHC-triphosphate is an intracellular metabolite of β-d-N4-Hydroxycytidine (NHC) as a triphosphate form. NHC-triphosphate is a weak alternative substrate for the viral polymerase and changes the mobility of the product in polyacrylamide electrophoresis gels[1]. |
|---|---|
| Related Catalog | |
| Target |
IC50: alternative substrate for the viral polymerase[1] |
| In Vitro | In an intracellular metabolism assay, HCV replicon cells are treated with 10 μM 3H-labeled NHC, and intracellular nucleotide levels are determined after 1, 2 and 8 hours incubations. NHC is rapidly convered into the mono-, di-, and triphosphate forms, and NHC-TP reaches up to 71.12 pM after 8 hours[1]. NHC-triphosphate (NHC-TP)(5-40 μM) absence leads to full-length polymerization products, it can be a weak alternative substrate. In addition, incorporation of NHC-TP instead of CTP increases the molecular weight of the polymerization product by 16 (one extra oxygen) for each event and an obvious electrophoretic shift is observed in cell-free HCV NS5B polymerization reactions[1]. |
| References |
| Molecular Formula | C9H16N3O15P3 |
|---|---|
| Molecular Weight | 499.15600 |
| Exact Mass | 498.97900 |
| PSA | 306.09000 |
| NHC-triphosphate |