Neurotensin (8-13) acetate salt

Modify Date: 2024-01-04 17:37:01

Neurotensin (8-13) acetate salt structure	Common Name	Neurotensin (8-13) acetate salt
	CAS Number	60482-95-3	Molecular Weight	816.99000
	Density	1.4 g/cm3	Boiling Point	N/A
	Molecular Formula	C₃₈H₆₄N₁₂O₈	Melting Point	N/A
	MSDS	USA	Flash Point	N/A

Use of Neurotensin (8-13) acetate salt

Neurotensin (8-13) is an active fragment of Neurotensin,. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.

Name	2-[[2-[[2-[[1-[2-[[2-amino-5-(diaminomethylideneamino)pentanoyl]amino]-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carbonyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-3-methylpentanoyl]amino]-4-methylpentanoic acid
Synonym	More Synonyms

Description	Neurotensin (8-13) is an active fragment of Neurotensin,. Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density.
Related Catalog	Signaling Pathways >> Others >> Others Research Areas >> Neurological Disease Peptides
Target	NTR1[1]
In Vitro	Receptor internalization induced by Neurotensin(8-13) results in a decrease in cell-surface NT1 receptors (NTR1) density. The receptor downregulation in response to high extracellular concentrations of the peptide has been described for Neurotensin (NT) in HT-29 cells and in rat primary cultured neurons. Reappearance of the receptors on the cell surface is also different[1].
Kinase Assay	Binding assays are performed on whole HT-29 cells at confluence. A day before the assay, cells (106 cells/0.4 mL, equivalent to 0.3 mg protein) are placed in 48-well plates. A special binding buffer that includes protease inhibitors (50 mM HEPES, 125 mM NaCl, 7.5 mM KCl, 5.5 mM MgCl2, 1 mM EGTA, 5 g/L bovine serum albumin, 2 mg/L chymostatin, 100 mg/L soybean trypsin inhibitor, 50 mg/L bacitracin, pH 7.4) is used for the experiments. In inhibition studies, cells are incubated for 1 h at 37°C in triplicate with 25,000 cpm of 125I-NT and variable concentrations (0.001-3,000 nM) of unlabeled NT(8-13), unlabeled NT-VIII, or NT-VIII labeled with natRe (final volume of 0.2 mL per well). The cells are then washed twice with cold binding buffer and afterward are solubilized with 1N NaOH at 37°C (0.4 mL per well). The activity is determined in a γ-counter. In saturation studies, cells are incubated in triplicate with increasing concentrations (0.1-10 nM) of 99mTc(CO)3NT-VIII for 1 h at 37°C (final volume, 0.2 mL per well). The concentrations of total technetium (99+99mTc) are equivalent to 0.2-20 MBq 99mTc activity per well. After 2 washings with the same binding buffer as before, the cells are then solubilized with 1N NaOH at 37°C (0.4 mL per well). The bound radioactivity is measured in the γ-counter. Nonspecific binding is determined with 1 μM unlabeled NT(8-13)[1].
References	[1]. García-Garayoa E, et al. Preclinical evaluation of a new, stabilized neurotensin(8--13) pseudopeptide radiolabeled with (99m)tc. J Nucl Med. 2002 Mar;43(3):374-83.

Density	1.4 g/cm3
Molecular Formula	C₃₈H₆₄N₁₂O₈
Molecular Weight	816.99000
Exact Mass	816.49700
PSA	344.06000
LogP	3.47920
Index of Refraction	1.642

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport

In vivo behavioral effects of stable, receptor-selective neurotensin[8-13] analogues that cross the blood-brain barrier.

Neuropharmacology 48(3) , 417-25, (2005)

A set of neurotensin[8-13] (NT[8-13]) analogues (KK1-19) has been evaluated in various pre-clinical assays relevant for further development of these compounds as potential antipsychotics. Initial scre...

The neurotensin fragment AcNT(8-13) inhibits lowering of interstitial fluid pressure in rat trachea.

Am. J. Physiol. Heart Circ. Physiol. 283(3) , H933-40, (2002)

Injury to soft tissue results in the lowering of interstitial fluid pressure (P(if)), plasma protein extravasation, and increased total tissue volume. In this study, the effects of N-acetyl neurotensi...

Radiolabeling of multimeric neurotensin(8-13) analogs with the short-lived positron emitter fluorine-18.

Appl. Radiat. Isot. 65(7) , 818-26, (2007)

Three methods for (18)F-labeling of dimeric and tetrameric neurotensin(8-13) derivatives were evaluated with respect to the labeling yield and the required peptide amounts. Labeling using N-succinimid...

Neurotensin(8-13)