C6 NBD Ceramide
Modify Date: 2024-01-11 19:19:51
C6 NBD Ceramide structure
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Common Name | C6 NBD Ceramide | ||
|---|---|---|---|---|
| CAS Number | 94885-02-6 | Molecular Weight | 575.740 | |
| Density | 1.2±0.1 g/cm3 | Boiling Point | N/A | |
| Molecular Formula | C30H49N5O6 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of C6 NBD CeramideC6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism[1][2][3]. |
| Name | c6-nbd-ceramide |
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| Synonym | More Synonyms |
| Description | C6 NBD Ceramide is a Golgi apparatus fluorescent probe with cell membrane permeability. C6 NBD Ceramide can be used for fast and convenient green fluorescent labeling of Golgi in living and fixed cells, and can be used to observe changes in Golgi morphology in living cells (Ex=466 nm, Em=536 nm). C6-NBD-ceramide is metabolized to fluorescent sphingomyelin and glucosylceramide, can be used for the study of sphingolipid transport and metabolic mechanism[1][2][3]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Golgi apparatus[3]: 1. Rinse cells (on glass cover slips) by HMEM, then fix cells in glutaraldehyde or paraformaldehyde for 5-10 min (25℃). 2. Wash fixed cells in a balanced salt solution (such as HCMF). 3. Transfer culture dishes containing the glass cover slips to an ice-water bath and incubate with freshly prepared NaBH4 for 3×5 min (for glutaraldehyde-fixed cells). 4. Rinse cells several times (20-30 min) in cold HCMF, warm to 25℃, then incubate with C6 NBD-ceramide/BSA for 60 min. 5. Wash fixed cells by HCMF, and incubate with 10% fetal calf serum or 2 mg/mL BSA (30-90 min; 25℃) to back-exchange excess C6 NBD-ceramide from the preparation and enhance the staining of the Golgi apparatus. 6. The glass cover slips mounted on depression slides and observe by fluorescence microscope (Ex=466 nm, Em=536 nm). |
| References |
[1]. Lipsky NG, et al. A vital stain for the Golgi apparatus. Science. 1985 May 10;228(4700):745-7. |
| Density | 1.2±0.1 g/cm3 |
|---|---|
| Molecular Formula | C30H49N5O6 |
| Molecular Weight | 575.740 |
| Exact Mass | 575.368286 |
| PSA | 180.32000 |
| LogP | 8.25 |
| Index of Refraction | 1.562 |
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| N-[(2S,3R,4E)-1,3-Dihydroxy-4-octadecen-2-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide |
| N-{6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl}sphingosine |
| Hexanamide, N-[(1S,2S,3E)-2-hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- |
| N-[(2S,3R,4E)-1,3-dihydroxyoctadec-4-en-2-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide |
| N-[(2S,3S,4E)-1,3-Dihydroxy-4-octadecen-2-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide |
| N-[(2S,3S,4E)-1,3-Dihydroxyoctadec-4-en-2-yl]-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanamide |