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AC-DEVD-AMC

AC-DEVD-AMC用途

Ac-DEVD-AMC 是 Caspase-3 的底物。

AC-DEVD-AMC名称

[ CAS 号 ]:
169332-61-0

[ 中文名 ]:
Ac-Asp-Glu-Val-Asp-7-氨基-4-甲基香豆素

[ 英文名 ]:
Ac-Asp-Glu-Val-Asp-AMC ammonium salt

[英文别名 ]:

Ac-DEVD-AMC
MFCD00671411

AC-DEVD-AMC生物活性

[ 描述 ]:

Ac-DEVD-AMC 是 Caspase-3 的底物。

[ 相关类别 ]:

信号通路 >> 其他 >> 其他

染料试剂

多肽产品

研究领域 >> 癌症

[体外研究]

Ac-DEVD-AMC是caspase-3底物,可用于监测细胞内caspase-3活性[1] .AMC部分最高2 h(面积= 5.6±1.8),Ac-DEVD的AMC峰面积-AMC在没有肺标本(单独底物)的情况下孵育6小时相对较小[2]。

[细胞实验]

从大鼠和小鼠收集肺片段（各约20mg）。将样品在37℃下在50mL KH缓冲液或MEM（连续气体与95％O 2：5％CO 2）中温育最多6小时。在特定时间点，将样品在含氧KH缓冲液或含有32μMzVAD-fmk或15μLDMSO的MEM中孵育20分钟（f / v = 1.0mL）。然后加入Ac-DEVD-AMC（37μM）并继续孵育20分钟。在孵育结束时，通过剧烈均质化2分钟破坏组织，超声处理3分钟并通过27-G针进行10次传代。该破坏程序由于稀释而淬灭了Ac-DEVD-AMC裂解反应。通过微量离心过滤器离心（~6,300g，90分钟）收集上清液，在HPLC上分离，并分析荧光AMC部分[2]。

[参考文献]

[1]. Ahmed R Alsuwaidi, et al. Bioenergetics of murine lungs infected with respiratory syncytial virus. Virol J. 2013; 10: 22.

[2]. Ahmed R Alsuwaidi, et al. Lung tissue bioenergetics and caspase activity in rodents. BMC Res Notes. 2013; 6: 12.

[相关活性小分子]

AC-DEVD-AMC物理化学性质

[ 密度 ]:
1.4±0.1 g/cm3

[ 沸点 ]:
1200.1±65.0 °C at 760 mmHg

[ 分子式 ]:
C₃₀H₃₇N₅O₁₃

[ 分子量 ]:
675.641

[ 闪点 ]:
679.6±34.3 °C

[ 精确质量 ]:
675.238770

[ PSA ]:
287.61000

[ LogP ]:
1.07

[ 外观性状 ]:
白色粉末

[ 蒸汽压 ]:
0.0±0.3 mmHg at 25°C

[ 折射率 ]:
1.594

[ 储存条件 ]:
−20°C

[ 水溶解性 ]:
DMSO: 10 mM

AC-DEVD-AMCMSDS

详细MSDS(安全技术说明书)

AC-DEVD-AMC安全信息

[ 个人防护装备 ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

AC-DEVD-AMC文献

RETRA exerts anticancer activity in Ewing's sarcoma cells independent of their TP53 status.

Eur. J. Cancer 51(7) , 841-51, (2015)

Mutant p53 can exert oncogenic activity by inhibitory interaction with p73. The small-molecule RETRA has been described to disrupt this interaction and to suppress carcinoma cells (Kravchenko et al., ...

Neuroprotection of atractylenolide III from Atractylodis macrocephalae against glutamate-induced neuronal apoptosis via inhibiting caspase signaling pathway.

Neurochem. Res. 39(9) , 1753-8, (2014)

Glutamate-induced excitotoxicity appears to play a crucial role in neurological disorders. Neuroprotection against glutamate-induced excitotoxicity has been proposed as a therapeutic strategy for prev...

Synergistic apoptosis-inducing effects on A375 human melanoma cells of natural borneol and curcumin.

PLoS ONE 9(6) , e101277, (2014)

This study was to investigate the synergistic effect of NB/Cur on growth and apoptosis in A375 human melanoma cell line by MTT assay, flow cytometry and Western blotting. Our results demonstrated that...

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