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去氧胆酸钠

去氧胆酸钠用途

Deoxycholic acid sodium salt 激活 G 蛋白偶联胆汁酸受体TGR5。

去氧胆酸钠名称

[ CAS 号 ]:
302-95-4

[ 中文名 ]:
脱氧胆酸钠

[ 英文名 ]:
Sodium deoxycholate

[中文别名 ]:

[英文别名 ]:

Sodium Choleate
desoxycholatesodium
EINECS 206-132-7
deoxycholatesodium
Sodium deoxycholate
SODIUM DESOXYCHOLATE
Deoxycholic acid,sodium salt
OXIDE EXTRACT
SODIUMDISOXYCHOLATE
na-desoxycholat

去氧胆酸钠生物活性

[ 描述 ]:

Deoxycholic acid sodium salt 激活 G 蛋白偶联胆汁酸受体TGR5。

[ 相关类别 ]:

信号通路 >> G 蛋白偶联受体/G 蛋白 >> GPCR19

研究领域 >> 代谢疾病

[ 靶点 ]

TGR5[1]

[体外研究]

脱氧胆酸(DCA)和chenoDeoxycholic acid(CDCA)作为十二指肠反流的常见成分,在许多生理和病理过程中起协同作用。将细胞在pH 5.5下反复暴露于100μMCDCA和脱氧胆酸至多120分钟。为了模拟体外慢性局部复发疾病,将胃癌细胞系MGC803暴露于含有100μM脱氧胆酸和CDCA的酸化培养基(pH5.5)中。产生未处理的对数生长MGC803细胞系以用作正常pH培养基中的对照。每天暴露于酸化的胆汁酸10分钟,持续60周后,MGC803抗性细胞在暴露120分钟后能够存活并增殖[2]。

[细胞实验]

MGC803细胞在Roswell Park Memorial Institute培养基中培养，所述培养基补充有10％胎牛血清和100U / mL青霉素和100mg / mL链霉素。为了产生MGC803抗性细胞，使用盐酸（A）将MGC803培养基的pH值调节至实验条件。用培养基将胆汁酸GCDA和脱氧胆酸稀释至100μM（B）的最佳工作浓度，并将总pH（A + B）调节至pH5.5，模拟胃环境。最初，MGC803细胞长期暴露于含有胆汁酸（A + B）的酸化培养基中，每24小时一次。在我们的初步实验中优化了实验时间和条件，其显示10分钟足够并且不会导致细胞损伤。重复该过程，MGC803细胞在A + B暴露120分钟后存活并增殖需要60周。对照未处理的细胞在pH7.4的中性RPMI培养基中与抗性细胞平行培养60周。在30和60周时，记录了暴露于酸化胆汁酸（A + B）的MGC803细胞的形态变化[2]。

[参考文献]

[1]. Somm E, et al. β-Klotho deficiency protects against obesity through a crosstalk between liver, microbiota, and brown adipose tissue. JCI Insight. 2017 Apr 20;2(8). pii: 91809.

[2]. Wang X, et al. Acidified bile acids enhance tumor progression and telomerase activity of gastric cancer in micedependent on c-Myc expression. Cancer Med. 2017 Apr;6(4):788-797.

[相关活性小分子]

INT-777 | INT-767 | 去氧胆酸 | BAR501 | 3-(2-氯苯基)-n-(4-氯苯基)-n,5-二甲基-4-异噁唑羧酰胺 | SB756050 | BAR502 | 猪去氧胆酸

去氧胆酸钠物理化学性质

[ 密度 ]:
1.128g/cm3

[ 沸点 ]:
547.1ºC at 760 mmHg

[ 熔点 ]:
357-365 °C

[ 分子式 ]:
C₂₄H₃₉NaO₄

[ 分子量 ]:
414.554

[ 精确质量 ]:
414.274597

[ PSA ]:
80.59000

[ LogP ]:
3.14320

[ 外观性状 ]:
奶油色结晶粉末

[ 储存条件 ]:
存放于惰性气体之中;避免湿气 (吸湿)

[ 水溶解性 ]:
330 g/L (15 ºC)

去氧胆酸钠MSDS

详细MSDS(安全技术说明书)

去氧胆酸钠毒性和生态

CHEMICAL IDENTIFICATION

RTECS NUMBER :: FZ2250000

CHEMICAL NAME :: 5-beta-Cholan-24-oic acid, 3-alpha,12-alpha-dihydroxy-, sodium salt

CAS REGISTRY NUMBER :: 302-95-4

LAST UPDATED :: 199701

DATA ITEMS CITED :: 20

MOLECULAR FORMULA :: C24-H39-O4.Na

MOLECULAR WEIGHT :: 414.62

WISWESSER LINE NOTATION :: L E5 B666TJ A1 DQ E1 FY1&2VO OQ &-NA-

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 1370 mg/kg

TOXIC EFFECTS :: Behavioral - altered sleep time (including change in righting reflex) Behavioral - ataxia Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 123 mg/kg

TOXIC EFFECTS :: Behavioral - altered sleep time (including change in righting reflex) Behavioral - ataxia Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 2430 mg/kg

TOXIC EFFECTS :: Behavioral - altered sleep time (including change in righting reflex) Behavioral - ataxia Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 150 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 1050 mg/kg

TOXIC EFFECTS :: Behavioral - somnolence (general depressed activity) Gastrointestinal - ulceration or bleeding from stomach Gastrointestinal - ulceration or bleeding from small intestine

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 36 mg/kg

TOXIC EFFECTS :: Behavioral - excitement Lungs, Thorax, or Respiration - other changes Gastrointestinal - hypermotility, diarrhea

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 815 mg/kg

TOXIC EFFECTS :: Behavioral - altered sleep time (including change in righting reflex) Behavioral - ataxia Lungs, Thorax, or Respiration - other changes

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 107 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 15 mg/kg

TOXIC EFFECTS :: Cardiac - other changes Lungs, Thorax, or Respiration - acute pulmonary edema Blood - other changes

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 700 mg/kg/28D-C

TOXIC EFFECTS :: Gastrointestinal - other changes Liver - changes in liver weight Lungs, Thorax, or Respiration - changes in lung weight

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Oral

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 6 gm/kg/30D-I

TOXIC EFFECTS :: Liver - changes in liver weight Biochemical - Enzyme inhibition, induction, or change in blood or tissue levels - transaminases Related to Chronic Data - death

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 1400 mg/kg

SEX/DURATION :: male 28 day(s) pre-mating

TOXIC EFFECTS :: Reproductive - Paternal Effects - prostate, seminal vesicle, Cowper's gland, accessory glands

MUTATION DATA

TEST SYSTEM :: Rodent - rat

DOSE/DURATION :: 10 umol/kg

REFERENCE :: GANNA2 Gann. Japanese Journal of Cancer Research. (Tokyo, Japan) V.1-75, 1907-84. For publisher information, see JJCREP. Volume(issue)/page/year: 75,29,1984 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOHS - National Occupational Hazard Survey (1974) NOHS Hazard Code - 83389 No. of Facilities: 138 (estimated) No. of Industries: 1 No. of Occupations: 2 No. of Employees: 361 (estimated) NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - 83389 No. of Facilities: 673 (estimated) No. of Industries: 6 No. of Occupations: 9 No. of Employees: 7419 (estimated) No. of Female Employees: 5995 (estimated)

去氧胆酸钠安全信息

[ 符号 ]:

GHS07

[ 信号词 ]:
Warning

[ 危害声明 ]:
H302-H335

[ 警示性声明 ]:
P301 + P312 + P330

[ 个人防护装备 ]:
dust mask type N95 (US);Eyeshields;Gloves

[ 危害码 (欧洲) ]:
Xn:Harmful;

[ 风险声明 (欧洲) ]:
R22

[ 安全声明 (欧洲) ]:
S22

[ 危险品运输编码 ]:
NONH for all modes of transport

[ WGK德国 ]:
3

[ RTECS号 ]:
FZ2250000

[ 海关编码 ]:
29181930

去氧胆酸钠上下游产品

去氧胆酸钠上游产品

去氧胆酸钠下游产品

去氧胆酸钠海关

[ 海关编码 ]: 29181930

去氧胆酸钠文献

Salicylic acid signaling controls the maturation and localization of the arabidopsis defense protein ACCELERATED CELL DEATH6.

Mol. Plant 7(8) , 1365-83, (2014)

ACCELERATED CELL DEATH6 (ACD6) is a multipass membrane protein with an ankyrin domain that acts in a positive feedback loop with the defense signal salicylic acid (SA). This study implemented biochemi...

Salicylic acid regulates Arabidopsis microbial pattern receptor kinase levels and signaling.

Plant Cell 26(10) , 4171-87, (2014)

In Arabidopsis thaliana, responses to pathogen-associated molecular patterns (PAMPs) are mediated by cell surface pattern recognition receptors (PRRs) and include the accumulation of reactive oxygen s...

Co-ordinated brain and craniofacial development depend upon Patched1/XIAP regulation of cell survival.

Hum. Mol. Genet. 24(3) , 698-713, (2015)

Congenital brain and craniofacial defects often occur together as a consequence of their developmental dependency on common progenitor tissue interactions and signaling pathways during embryogenesis. ...

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