256411-32-2
256411-32-2 structure
- Name: Belotecan
- Chemical Name: Camtobell
- CAS Number: 256411-32-2
- Molecular Formula: C25H27N3O4
- Molecular Weight: 433.500
- Catalog: Signaling Pathways Apoptosis Apoptosis
- Create Date: 2018-10-23 18:32:02
- Modify Date: 2024-01-02 12:04:13
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Belotecan (CKD-602 free base) is a DNA topoisomerase I inhibitor. Belotecan induces cell apoptosis and cell-cycle arrest. Belotecan is a camptothecin analogue with anti-tumor effects, it can be used for the research of cancer[1].
| Name | Camtobell |
|---|---|
| Synonyms |
Belotecan
7-(2-(N-Isopropylamino)ethyl)camptothecin (4S)-4-Ethyl-4-hydroxy-11-[2-(isopropylamino)ethyl]-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione Belotecan [INN] CKD602 UNII-27Z82M2G1N (4S)-4-Ethyl-4-hydroxy-11-(2-(isopropylamino)ethyl)-1,12-dihydro-14H-pyrano(3',4':6,7)indolizino(1,2-b)quinoline-3,14(4H)-dione 1H-Pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione, 4-ethyl-4-hydroxy-11-[2-[(1-methylethyl)amino]ethyl]-, (4S)- |
| Description | Belotecan (CKD-602 free base) is a DNA topoisomerase I inhibitor. Belotecan induces cell apoptosis and cell-cycle arrest. Belotecan is a camptothecin analogue with anti-tumor effects, it can be used for the research of cancer[1]. |
|---|---|
| Related Catalog | |
| Target |
IC50: 30 ng/mL (Caski cells), 150 ng/mL (HeLa cells), 150 ng/mL (SiHa cells)[1] |
| In Vitro | Belotecan (0-600 ng/mL; 0-72 h) time- and dose-dependently inhibits viability of Caski cells, HeLa cells and SiHa cells at 48 h with IC50 values of 30, 150 and 150 ng/mL, respectively[1]. Belotecan (0-150 ng/mL; 48 h) induces cell apoptosis and cell-cycle arrest, and affects PARP, cleaved PARP, BAX, p53, Ser15, cell cycle related protein expression and cancer invasion in cervical cancer[1]. Apoptosis Analysis[1] Cell Line: Caski, HeLa and SiHa cell lines Concentration: 0-150 ng/mL Incubation Time: 48 h Result: Showed strong pro-apoptotic activities to Caski, HeLa and SiHa cells. Western Blot Analysis[1] Cell Line: Caski, HeLa and SiHa cell lines Concentration: 0-150 ng/mL Incubation Time: 48 h Result: Increased the expression of PARP, cleaved PARP, BAX, p53, Ser15, cyclin B1, phosphorylated cyclin B1 and phospho-cdc2 (Tyr15) protein, and decreased MMP2 and VEGF protein expression. Cell Cycle Analysis[1] Cell Line: Caski, HeLa and SiHa cell lines Concentration: 0-150 ng/mL Incubation Time: 48 h Result: Induced cell-cycle arrest in the G2/M phase in cervical cancer. Cell Invasion Assay[1] Cell Line: Caski, HeLa and SiHa cell lines Concentration: 0-150 ng/mL Incubation Time: 48 h Result: Inhibited cancer invasion in cervical cancer. |
| In Vivo | Belotecan (25 mg/kg; i.v. for 16 days at 4-day intervals) inhibits tumor growth in CaSki-xenografts nude mice[1]. Animal Model: BALB/c-nude mice with CaSki cells injection[1] Dosage: 25 mg/kg Administration: Intravenous injection; 25 mg/kg; 16 days at 4-day intervals Result: Significantly inhibited the tumor growth and showed no significant difference in bodyweight of xenograft mice and the controls. |
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 772.4±60.0 °C at 760 mmHg |
| Molecular Formula | C25H27N3O4 |
| Molecular Weight | 433.500 |
| Flash Point | 420.9±32.9 °C |
| Exact Mass | 433.200165 |
| PSA | 93.45000 |
| LogP | 1.86 |
| Vapour Pressure | 0.0±2.8 mmHg at 25°C |
| Index of Refraction | 1.679 |